Serum albumin-coated bone allograft (BoneAlbumin) results in faster bone formation and mechanically stronger bone in aging rats

Dénes B. Horváthy, Károly Schandl, Charlotte M. Schwarz, Károly Renner, István Hornyák, Bence T. Szabó, Eugenia Niculescu-Morzsa, Stefan Nehrer, Csaba Dobó-Nagy, Attila Doros, Z. Lacza

Research output: Article

Abstract

Serum albumin-coated bone allografts (BoneAlbumin) have successfully supported bone regeneration in various experimental models by activating endogenous progenitors. However, the effect of tissue aging, linked to declining stem cell function, has yet to be explicitly examined within the context of BoneAlbumin's regenerative capacity. Stem cell function was tested with an in vitro attachment assay, which showed that albumin coating increases stem cell attachment on demineralized bone surfaces in an aging cell population. Bone regeneration was investigated in vivo by creating critical size bone defects on the parietal bones of aging female rats. Demineralized bone matrices with and without serum albumin coating were used to fill the defects. Bone regeneration was determined by measuring the density and the size of the remaining bone defect with computed tomography (CT). Microcomputed tomography (MicroCT) and mechanical testing were performed on the parietal bone explants. In vivo CT and ex vivo microCT measurements showed better regeneration with albumin-coated grafts. Additionally, the albumin-coated group showed a twofold increase in peak fracture force compared with uncoated allografts. In the present study, serum albumin-coated demineralized bone matrices successfully supported faster and functionally superior bone regeneration in aging rats. Because stem cell function, a key contributor of bone remodelling, decreases with age and serum albumin is an effective activator of endogenous progenitor cells, this method could be an effective and safe adjuvant in bone regeneration of aging adult and osteo-compromised populations.

Original languageEnglish
JournalJournal of Tissue Engineering and Regenerative Medicine
DOIs
Publication statusPublished - jan. 1 2019

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Bone Regeneration
Osteogenesis
Serum Albumin
Allografts
Rats
Bone
Stem Cells
Aging of materials
Bone and Bones
Parietal Bone
Albumins
X-Ray Microtomography
Bone Matrix
Tomography
Stem cells
Bone Remodeling
Cell Aging
Population
Regeneration
Theoretical Models

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Serum albumin-coated bone allograft (BoneAlbumin) results in faster bone formation and mechanically stronger bone in aging rats. / Horváthy, Dénes B.; Schandl, Károly; Schwarz, Charlotte M.; Renner, Károly; Hornyák, István; Szabó, Bence T.; Niculescu-Morzsa, Eugenia; Nehrer, Stefan; Dobó-Nagy, Csaba; Doros, Attila; Lacza, Z.

In: Journal of Tissue Engineering and Regenerative Medicine, 01.01.2019.

Research output: Article

Horváthy, Dénes B. ; Schandl, Károly ; Schwarz, Charlotte M. ; Renner, Károly ; Hornyák, István ; Szabó, Bence T. ; Niculescu-Morzsa, Eugenia ; Nehrer, Stefan ; Dobó-Nagy, Csaba ; Doros, Attila ; Lacza, Z. / Serum albumin-coated bone allograft (BoneAlbumin) results in faster bone formation and mechanically stronger bone in aging rats. In: Journal of Tissue Engineering and Regenerative Medicine. 2019.
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AU - Renner, Károly

AU - Hornyák, István

AU - Szabó, Bence T.

AU - Niculescu-Morzsa, Eugenia

AU - Nehrer, Stefan

AU - Dobó-Nagy, Csaba

AU - Doros, Attila

AU - Lacza, Z.

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