Serpins and the complement system

László Beinrohr, Thomas A. Murray-Rust, Leanne Dyksterhuis, P. Závodszky, P. Gál, Robert N. Pike, Lakshmi C. Wijeyewickrema

Research output: Article

8 Citations (Scopus)

Abstract

C1-inhibitor (serpin G1) is a 105 kDa inhibitor which functions as a major antiinflammatory protein in the body. It has its effects via inhibition of the proteases of the complement system and contact system of coagulation, as well as several direct effects mediated by its unique highly glycosylated N-terminal domain. The serpin controls a number of different proteases very efficiently and for some of these the function is augmented by the cofactor, heparin. Here, we describe the preparation of human plasma and recombinant C1-inhibitor and the basic methods required for their characterization, using the complement enzyme C1s as an example of a target enzyme.

Original languageEnglish
Pages (from-to)55-75
Number of pages21
JournalMethods in Enzymology
Volume499
DOIs
Publication statusPublished - 2011

Fingerprint

Serpins
Complement C1s
Peptide Hydrolases
Complement C1 Inhibitor Protein
Plasma (human)
Enzymes
Coagulation
Heparin
Anti-Inflammatory Agents
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Beinrohr, L., Murray-Rust, T. A., Dyksterhuis, L., Závodszky, P., Gál, P., Pike, R. N., & Wijeyewickrema, L. C. (2011). Serpins and the complement system. Methods in Enzymology, 499, 55-75. https://doi.org/10.1016/B978-0-12-386471-0.00004-3

Serpins and the complement system. / Beinrohr, László; Murray-Rust, Thomas A.; Dyksterhuis, Leanne; Závodszky, P.; Gál, P.; Pike, Robert N.; Wijeyewickrema, Lakshmi C.

In: Methods in Enzymology, Vol. 499, 2011, p. 55-75.

Research output: Article

Beinrohr, L, Murray-Rust, TA, Dyksterhuis, L, Závodszky, P, Gál, P, Pike, RN & Wijeyewickrema, LC 2011, 'Serpins and the complement system', Methods in Enzymology, vol. 499, pp. 55-75. https://doi.org/10.1016/B978-0-12-386471-0.00004-3
Beinrohr, László ; Murray-Rust, Thomas A. ; Dyksterhuis, Leanne ; Závodszky, P. ; Gál, P. ; Pike, Robert N. ; Wijeyewickrema, Lakshmi C. / Serpins and the complement system. In: Methods in Enzymology. 2011 ; Vol. 499. pp. 55-75.
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