Sequence heterogeneity among human picobirnaviruses detected in a gastroenteritis outbreak

Research output: Article

56 Citations (Scopus)

Abstract

Human picobirnaviruses characterised in this study were serendipitously detected in a non-bacterial gastroenteritis outbreak when specimens were examined for the presence of human rotaviruses using polyacrylamide gel electrophoresis. Often stool samples sent for virological examination, two, three, and one specimens were positive for human caliciviruses, picobirnaviruses, and both viruses, respectively. Partial sequences of the RNA-dependent RNA polymerase gene were determined for three picobirnavirus- positive samples. The sequence identity among these three strains was 60% to 65% for the nucleic acid and 64% to 70% for the deduced amino acid sequences. Phylogenetic analysis revealed that each of the three strains clustered with strains identified in geographically separate areas. In contrast, human calicivirus strains co-incidentally identified, showed complete nucleotide sequence identity. These findings demonstrate a lack of common exposure to or point of source for picobirnavirus infection, suggesting that the outbreak was caused by human caliciviruses. Further studies are needed to determine the etiologic role and to establish the taxonomic basis of picobirnaviruses.

Original languageEnglish
Pages (from-to)2281-2291
Number of pages11
JournalArchives of virology
Volume148
Issue number12
DOIs
Publication statusPublished - dec. 1 2003

    Fingerprint

ASJC Scopus subject areas

  • Virology

Cite this