Separation of ascorbigen, 1′-methylascorbigen, and their derivatives by liquid chromatographic techniques. Part 1. Thin layer chromatography

György Kátay, Erno Tyihák, Gyula Szókán

Research output: Article

9 Citations (Scopus)

Abstract

Methods are described for the efficient separation of ascorbigen, 1′-methylascorbigen, and other possible molecules with different substituants on the indole and ascorbic acid skeleton by normal and reversed phase thin layer chromatography. The UV spectra of ascorbigen and 1′-methylascorbigen obtained by spectrophotometric measurement were compared with those obtained by in situ spectroscopy on the sorbent layer. The detection limits also were determined for ascorbigen and 1′-methylascorbigen using different methods of visualization and densitometric evaluation of the spots or bands.

Original languageEnglish
Pages (from-to)98-102
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume9
Issue number2
Publication statusPublished - márc. 1 1996

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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