Separation of active and inactive (nonphosphorylating) Ca2+-ATPase in sacroplasmic reticulum subfractions from low-frequency-stimulated rabbit muscle

Shigeki Matsushita, László Dux, Dirk Pette

Research output: Article

11 Citations (Scopus)


Chronic low-frequency stimulation elicits in rabbit fast-twitch muscle a partial inactivation of the sarcoplasmic reticulum (SR) Ca2+-ATPase and Ca2+-uptake activities. Inactive Ca2+-ATPase was enriched in a light microsomal fraction by sucrose density gradient centrifugation after calcium oxalate loading in the presence of ATP. This fraction showed a reduced specific activity and phosphoprotein formation of the Ca2+-transport ATPase. These results suggest that the inactivation of the Ca2+-ATPase as induced by increased contractile activity, is confined to a specific SR vesicle population.

Original languageEnglish
Pages (from-to)203-206
Number of pages4
JournalFEBS letters
Issue number3
Publication statusPublished - dec. 9 1991


ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this