Sensitivity enhancement for mycotoxin determination by optical waveguide lightmode spectroscopy using gold nanoparticles of different size and origin

N. Adányi, Ádám György Nagy, Bettina Takács, István Szendrő, George Szakacs, Rózsa Szűcs, Eszter Tóth-Szeles, I. Lagzi, Diána Weiser, Viktória Bódai, Péter Sátorhelyi, Balázs Erdélyi

Research output: Article

3 Citations (Scopus)

Abstract

Mycotoxins, present in a wide range of food and feed commodities, are toxic secondary metabolites produced by a number of different fungi. Certain mycotoxins do not readily degrade at high temperatures, therefore are resistant to food processing, and consequently are present in the human and animal food supply. Optical waveguide lightmode spectroscopy (OWLS) was applied for the detection of aflatoxin B1, in a competitive immunoassay format, to compare the analytical sensitivity achieved with an immunosensor design allowing signal enhancement by increasing the sensor surface through immobilization of gold nanoparticles (AuNPs) of different size and origin (obtained by chemical or biotechnological synthesis). The effects of AuNPs median size, the methods of sensitization and the biochemical parameters on immunosensor performace were examined. After optimization of the sensitized sensor surface, an immunosensing method was developed for the analysis of aflatoxin in paprika matrix and the results were compared with HPLC reference measurements.

Original languageEnglish
JournalFood Chemistry
DOIs
Publication statusAccepted/In press - jan. 1 2018

Fingerprint

Immunosensors
Mycotoxins
Optical waveguides
Gold
Nanoparticles
mycotoxins
sensors (equipment)
spectroscopy
Spectrum Analysis
Spectroscopy
Food supply
paprika
Capsicum
Food processing
Food Handling
Aflatoxin B1
Aflatoxins
Food Supply
Poisons
Sensors

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

Sensitivity enhancement for mycotoxin determination by optical waveguide lightmode spectroscopy using gold nanoparticles of different size and origin. / Adányi, N.; Nagy, Ádám György; Takács, Bettina; Szendrő, István; Szakacs, George; Szűcs, Rózsa; Tóth-Szeles, Eszter; Lagzi, I.; Weiser, Diána; Bódai, Viktória; Sátorhelyi, Péter; Erdélyi, Balázs.

In: Food Chemistry, 01.01.2018.

Research output: Article

Adányi, N. ; Nagy, Ádám György ; Takács, Bettina ; Szendrő, István ; Szakacs, George ; Szűcs, Rózsa ; Tóth-Szeles, Eszter ; Lagzi, I. ; Weiser, Diána ; Bódai, Viktória ; Sátorhelyi, Péter ; Erdélyi, Balázs. / Sensitivity enhancement for mycotoxin determination by optical waveguide lightmode spectroscopy using gold nanoparticles of different size and origin. In: Food Chemistry. 2018.
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