Sebaceous Gland-Rich Skin Is Characterized by TSLP Expression and Distinct Immune Surveillance Which Is Disturbed in Rosacea

Zsolt Dajnoki, Gabriella Béke, A. Kapitány, Gábor Mócsai, Krisztián Gáspár, Ralph Rühl, Zoltán Hendrik, I. Juhász, Christos C. Zouboulis, Attila Bácsi, T. Bíró, D. Törőcsik, A. Szegedi

Research output: Article

11 Citations (Scopus)

Abstract

The microbial community exhibits remarkable diversity on topographically distinct skin regions, which may be accompanied by differences in skin immune characteristics. Our aim was to compare the immune milieu of healthy sebaceous gland-rich (SGR) and sebaceous gland-poor skin areas, and to analyze its changes in an inflammatory disease of SGR skin. For this purpose, immunohistochemical, immunocytochemical, and quantitative real-time PCR analyses of thymic stromal lymphopoietin (TSLP) and other cytokines, phenotypic immune cell markers and transcription factors were carried out in samples from sebaceous gland-poor, SGR skin and from papulopustular rosacea. TSLP mRNA and protein production was also studied in cultured keratinocytes. In SGR skin, higher TSLP expression, dendritic cell appearance without prominent activation, and T cell presence with IL-17/IL-10 cytokine milieu were detected compared with sebaceous gland-poor skin. Linoleic acid, a major sebum component, was found to induce TSLP expression dose-dependently in keratinocytes. In papulopustular rosacea, significantly decreased TSLP level and influx of inflammatory dendritic cells and T cells with IL-17/interferon-γ cytokine milieu were observed. According to our results, SGR skin is characterized by a distinct, noninflammatory immune surveillance, which may explain the preferred localization of inflammatory skin diseases, and can influence future barrier repair therapeutic concepts.

Original languageEnglish
Pages (from-to)1114-1125
Number of pages12
JournalJournal of Investigative Dermatology
Volume137
Issue number5
DOIs
Publication statusPublished - máj. 1 2017

Fingerprint

Rosacea
Sebaceous Glands
Skin
Interleukin-17
Sebaceous Gland Diseases
T-cells
Cytokines
Keratinocytes
Dendritic Cells
Sebum
T-Lymphocytes
thymic stromal lymphopoietin
Linoleic Acid
Skin Diseases
Interleukin-10
Interferons
Real-Time Polymerase Chain Reaction
Transcription Factors
Biomarkers
Repair

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Sebaceous Gland-Rich Skin Is Characterized by TSLP Expression and Distinct Immune Surveillance Which Is Disturbed in Rosacea. / Dajnoki, Zsolt; Béke, Gabriella; Kapitány, A.; Mócsai, Gábor; Gáspár, Krisztián; Rühl, Ralph; Hendrik, Zoltán; Juhász, I.; Zouboulis, Christos C.; Bácsi, Attila; Bíró, T.; Törőcsik, D.; Szegedi, A.

In: Journal of Investigative Dermatology, Vol. 137, No. 5, 01.05.2017, p. 1114-1125.

Research output: Article

Dajnoki, Zsolt ; Béke, Gabriella ; Kapitány, A. ; Mócsai, Gábor ; Gáspár, Krisztián ; Rühl, Ralph ; Hendrik, Zoltán ; Juhász, I. ; Zouboulis, Christos C. ; Bácsi, Attila ; Bíró, T. ; Törőcsik, D. ; Szegedi, A. / Sebaceous Gland-Rich Skin Is Characterized by TSLP Expression and Distinct Immune Surveillance Which Is Disturbed in Rosacea. In: Journal of Investigative Dermatology. 2017 ; Vol. 137, No. 5. pp. 1114-1125.
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