Screen-printed amperometric microcell for proline iminopeptidase enzyme activity assay

Géza Nagy, Robert E. Gyurcsányi, Alessandra Cristalli, Michael R. Neuman, Ernö Lindner

Research output: Article

13 Citations (Scopus)

Abstract

A microfabricated amperometric microcell was designed and used for the determination of proline iminopeptidase (PIP) enzyme activity in 2-10-μl samples. The measurements were made in the range of 10.3-841.5 mU/ml enzyme activities. The sensitivity of the determinations was between -0.0195 and -0.0203 μA ml/mU per min. The coefficient of variation of the determined values ranged between 2.8 (at 561.2 mU/ml) and 24.1% (at 10.3 mU/ml). The microcell was manufactured on an alumina substrate using screen-printed graphite working and Ag/AgCl reference electrodes. Elevated PIP activity in the vaginal fluid is a biochemical indicator of bacterial vaginosis. The method is appropriate to differentiate between normal (66±145 mU/ml) and elevated, diseased (704±145 mU/ml), values. Copyright (C) 2000 Elsevier Science S.A.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalBiosensors and Bioelectronics
Volume15
Issue number5-6
DOIs
Publication statusPublished - aug. 1 2000

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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