Scanning electron microscopic (SEM) analyses of cultured human embryo fibroblasts were performed 10 minutes, 1, 4 and 24 hours after X-irradiation with 2.5 Gy. Marked surface changes were observed 10 minutes and 1 hour after irradiation. These include the appearances of filopodia and lamellipodia as well as of plasma bridges connecting the detached membranes with the substrate. Initial signs of surface restoration, however, could be observed 4 hours after irradiation, while the full restoration of the confluent monolayer seemed to be completed at 24 hours. These observations as well as those obtained 1 hour after smaller and larger doses than above, i.e. 0.25 and 5 Gy, suggest that the early and temporary functional membrane alterations demonstrated previously by lectin-binding technique are in good agreement with well-defined micromorphological phenomena.
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Health, Toxicology and Mutagenesis