Sarcoplasmic reticulum (SR) Ca2+-ATPase as a marker of muscle cell differentiation: Immunohistochemical investigations of rhabdomyosarcomas and enhancement of the immunostaining after sodium methoxide pretreatment

T. Krenács, D. Stiller, L. Krenács, H. Bahn, E. Molnar, L. Dux

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Abstract

An immunocytochemical investigation of sarcoplasmic reticulum (SR) Ca2+-ATPase (SR-Ca-ATPase) was performed on formalin-fixed paraffin-embedded specimens of different types of rhabdomyosarcomas such as variants of embryonal and pleomorphic forms. Immunostaining frequency of tumours using SR-Ca-ATPase was compared with that of traditionally used muscle specific markers myoglobin, and desmin. Utilizing the possible cleaving of ester bounds sodium methoxide pretreatment was found to be very effective in enhancement of SR-Ca-ATPase immunostaining reaction. In 11 of 15 tissue specimens of 5 cases round shaped and elongated rhabdomyoblasts with definite cytoplasm exhibited positive immunoreactions with all of the polyclonal antibodies tested, using the streptavidin-biotinylated peroxidase complex (S-ABC-method). In formalin-fixed and paraffin-embedded material of 2 cases of undifferentiated rhabdomyosarcomas composed of small round tumour cells with scanty cytoplasm pretreatment with sodium methoxide induced the immunostaining of SR-Ca-ATPase. After that pretreatment a staining of the paranuclear cytoplasm occurred in many of these undifferentiated tumour cells. In these 2 cases, neither myoglobin nor desmin antibodies could react, However, when frozen sections of one of the poorly differentiated tumours were used monoclonal and polyclonal desmin antibodies reacted immunocytochemically in all of the small cells. Sodium methoxide induced or enhanced SR-Ca-ATPase immunocytochemical reaction can be a further addition to the diagnosis of rhabdomyosarcomas in formalin-fixed paraffin-embedded sections, even when desmin antibody fails to react.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalActa Histochemica
Volume88
Issue number2
Publication statusPublished - 1990

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Rhabdomyosarcoma
Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Desmin
Muscle Cells
Methanol
Cell Differentiation
Adenosine Triphosphatases
Paraffin
Formaldehyde
Cytoplasm
Antibodies
Myoglobin
Neoplasms
Streptavidin
Frozen Sections
Peroxidase
Esters
Staining and Labeling
Muscles

ASJC Scopus subject areas

  • Cell Biology

Cite this

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title = "Sarcoplasmic reticulum (SR) Ca2+-ATPase as a marker of muscle cell differentiation: Immunohistochemical investigations of rhabdomyosarcomas and enhancement of the immunostaining after sodium methoxide pretreatment",
abstract = "An immunocytochemical investigation of sarcoplasmic reticulum (SR) Ca2+-ATPase (SR-Ca-ATPase) was performed on formalin-fixed paraffin-embedded specimens of different types of rhabdomyosarcomas such as variants of embryonal and pleomorphic forms. Immunostaining frequency of tumours using SR-Ca-ATPase was compared with that of traditionally used muscle specific markers myoglobin, and desmin. Utilizing the possible cleaving of ester bounds sodium methoxide pretreatment was found to be very effective in enhancement of SR-Ca-ATPase immunostaining reaction. In 11 of 15 tissue specimens of 5 cases round shaped and elongated rhabdomyoblasts with definite cytoplasm exhibited positive immunoreactions with all of the polyclonal antibodies tested, using the streptavidin-biotinylated peroxidase complex (S-ABC-method). In formalin-fixed and paraffin-embedded material of 2 cases of undifferentiated rhabdomyosarcomas composed of small round tumour cells with scanty cytoplasm pretreatment with sodium methoxide induced the immunostaining of SR-Ca-ATPase. After that pretreatment a staining of the paranuclear cytoplasm occurred in many of these undifferentiated tumour cells. In these 2 cases, neither myoglobin nor desmin antibodies could react, However, when frozen sections of one of the poorly differentiated tumours were used monoclonal and polyclonal desmin antibodies reacted immunocytochemically in all of the small cells. Sodium methoxide induced or enhanced SR-Ca-ATPase immunocytochemical reaction can be a further addition to the diagnosis of rhabdomyosarcomas in formalin-fixed paraffin-embedded sections, even when desmin antibody fails to react.",
author = "T. Kren{\'a}cs and D. Stiller and L. Kren{\'a}cs and H. Bahn and E. Molnar and L. Dux",
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T1 - Sarcoplasmic reticulum (SR) Ca2+-ATPase as a marker of muscle cell differentiation

T2 - Immunohistochemical investigations of rhabdomyosarcomas and enhancement of the immunostaining after sodium methoxide pretreatment

AU - Krenács, T.

AU - Stiller, D.

AU - Krenács, L.

AU - Bahn, H.

AU - Molnar, E.

AU - Dux, L.

PY - 1990

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N2 - An immunocytochemical investigation of sarcoplasmic reticulum (SR) Ca2+-ATPase (SR-Ca-ATPase) was performed on formalin-fixed paraffin-embedded specimens of different types of rhabdomyosarcomas such as variants of embryonal and pleomorphic forms. Immunostaining frequency of tumours using SR-Ca-ATPase was compared with that of traditionally used muscle specific markers myoglobin, and desmin. Utilizing the possible cleaving of ester bounds sodium methoxide pretreatment was found to be very effective in enhancement of SR-Ca-ATPase immunostaining reaction. In 11 of 15 tissue specimens of 5 cases round shaped and elongated rhabdomyoblasts with definite cytoplasm exhibited positive immunoreactions with all of the polyclonal antibodies tested, using the streptavidin-biotinylated peroxidase complex (S-ABC-method). In formalin-fixed and paraffin-embedded material of 2 cases of undifferentiated rhabdomyosarcomas composed of small round tumour cells with scanty cytoplasm pretreatment with sodium methoxide induced the immunostaining of SR-Ca-ATPase. After that pretreatment a staining of the paranuclear cytoplasm occurred in many of these undifferentiated tumour cells. In these 2 cases, neither myoglobin nor desmin antibodies could react, However, when frozen sections of one of the poorly differentiated tumours were used monoclonal and polyclonal desmin antibodies reacted immunocytochemically in all of the small cells. Sodium methoxide induced or enhanced SR-Ca-ATPase immunocytochemical reaction can be a further addition to the diagnosis of rhabdomyosarcomas in formalin-fixed paraffin-embedded sections, even when desmin antibody fails to react.

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