Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population

Éva Csősz, Bernadett Márkus, Z. Darula, Katalin F. Medzihradszky, Judit Nemes, Emese Szabó, J. Tőzsér, C. Kiss, I. Márton

Research output: Article

2 Citations (Scopus)

Abstract

Oral squamous cell carcinoma (OSCC) is the seventh most common malignancy and the ninth most frequent cause of cancer death in Europe. Within Europe, Hungary has one of the highest rates of OSCC incidence and mortality. Thus, there is an urgent need to improve early detection. Saliva, as a readily available body fluid, became an increasingly important substance for the detection of biomarkers for many diseases. Different research groups have identified salivary biomarkers specific for OSCC for different countries. In this study, saliva samples of Hungarian patients with OSCC were studied to discover disease-specific and perhaps region-specific biomarkers. LC-mass spectrometry (MS)/MS analysis on a linear ion trap-Orbitrap mass spectrometer was used for qualitative and quantitative salivary protein profiling. More than 500 proteins were identified from saliva by shotgun proteomics. The up- and downregulated proteins in the saliva of patients with OSCC highlighted the importance of protein–protein interaction networks involving the immune system and proteolysis in disease development. Two potential biomarkers from our shotgun analysis and a third candidate reported earlier by a Taiwanese group were further examined by ELISA on a larger reference set of samples. Resistin, a biomarker reported in Taiwan but not validated in our study, highlights the necessity of application of standardized analysis methods in different ethnic or geographical populations to identify biomarkers with sufficient specificity and sensitivity.

Original languageEnglish
Pages (from-to)556-569
Number of pages14
JournalFEBS Open Bio
Volume8
Issue number4
DOIs
Publication statusPublished - ápr. 1 2018

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Biomarkers
Proteome
Squamous Cell Carcinoma
Saliva
Population
Firearms
Mass spectrometry
Proteolysis
Salivary Proteins and Peptides
Resistin
Hungary
Immune system
Body fluids
Body Fluids
Mass spectrometers
Tandem Mass Spectrometry
Taiwan
Proteomics
Epithelial Cells
Cause of Death

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population. / Csősz, Éva; Márkus, Bernadett; Darula, Z.; Medzihradszky, Katalin F.; Nemes, Judit; Szabó, Emese; Tőzsér, J.; Kiss, C.; Márton, I.

In: FEBS Open Bio, Vol. 8, No. 4, 01.04.2018, p. 556-569.

Research output: Article

Csősz, Éva ; Márkus, Bernadett ; Darula, Z. ; Medzihradszky, Katalin F. ; Nemes, Judit ; Szabó, Emese ; Tőzsér, J. ; Kiss, C. ; Márton, I. / Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population. In: FEBS Open Bio. 2018 ; Vol. 8, No. 4. pp. 556-569.
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