BACKGROUND. Integrins participate in cell-cell and cell-matrix interactions. In this study we determined whether αIIbβ3 integrin is involved in metastasis of human prostate adenocarcinoma cells. METHODS. Prostate adenocarcinoma PC-3 and DU-145 cell lines express αIIbβ3. Northern blotting, 5'-RACE, and immunofluorescent localization confirmed expression of αIIb integrin in prostate adenocarcinoma cells. We used orthotopic/ectopic site of implantation and lung colonization assays in SCID mice to determine whether aIIbβ3 participates in metastatasis of tumor cells. RESULTS. Immunofluorescent localization of αIIb integrin in fibronectin-adherent DU- 145 and PC-3 cells is remarkably different. In DU-145 cells the integrin localizes to focal contact sites, whereas it is predominantly intracellular in PC-3 cells. Both tumor cell lines are tumorigenic when implanted subcutaneously or intraprostatically in SCID mice, but only DU-145 cells injected intraprostatically metastasize. Flow cytometry with a mAb directed to αIIbβ3 revealed higher expression of αIIbβ3 in DU-145 tumor cell suspensions isolated from the prostate when compared to DU-145 tumor cells from the subcutis. Function-blocking mAbs to αIIbβ3 inhibit lung colonization of tail vein-injected DU-145 cells. CONCLUSIONS. Altogether, the data suggest that αIIbβ3 integrin participates in the metastatic progression of prostatic adenocarcinoma.
|Number of pages||8|
|Publication status||Published - máj. 15 1998|
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