Role for serotonin3 receptors in the control of adrenocorticotropic hormone release from rat pituitary cell cultures

A. E. Calogero, G. Bagdy, N. Burrello, P. Polosa, R. D'Agata

Research output: Article

28 Citations (Scopus)

Abstract

Although several serotonin (5-HT) receptor types have been shown capable of stimulating the release of adrenocorticotropic hormone (ACTH) from the pituitary gland, relatively little is known about the role of the 5-HT3 receptor, a receptor that has generated a great deal of interest for its involvement in many behavioral and therapeutic effects. Hence, in this study, we tested the effects of the 5-HT(3/4) receptor antagonist 3-tropanyl-indole-3-carboxylate (ICS 205-930) and the selective 5-HT3 receptor antagonist 3-tropanyl-3,5-dichlorobenzoate (MDL 72222) on ACTH release stimulated by 5-HT from primary cultures of rat pituitary cells. Subsequently, we evaluated the effects of the selective 5-HT3 receptor agonist 1-(m-chlorophenyl)-biguanide (m-CPBG) on basal, corticotropin-releasing hormone (CRH)- and arginine vasopressin (AVP)-stimulated ACTH release. The maximal stimulatory effect of 5-HT (10-9 mol/l) on ACTH: release was antagonized by both ICS 205-930 and MDL 72222, suggesting that 5-HT stimulates basal ACTH release through activation of 5-HT3 receptors. Accordingly, m-CPBG stimulated basal ACTH release in a concentration-dependent fashion. In contrast to 5-HT, m-CPBG did not have any effect on CRH-stimulated ACTH release and inhibited AVP-stimulated ACTH release in a concentration-dependent manner. These data suggest that the 5-HT3 receptor is involved in the release of ACTH from the pituitary gland in vitro.

Original languageEnglish
Pages (from-to)251-254
Number of pages4
JournalEuropean Journal of Endocrinology
Volume133
Issue number2
Publication statusPublished - 1995

Fingerprint

Adrenocorticotropic Hormone
Cell Culture Techniques
Receptors, Serotonin, 5-HT3
tropisetron
Serotonin
Biguanides
Arginine Vasopressin
Corticotropin-Releasing Hormone
Pituitary Gland
Serotonin 5-HT3 Receptor Agonists
Serotonin 5-HT3 Receptor Antagonists
Serotonin Receptors
Therapeutic Uses

ASJC Scopus subject areas

  • Endocrinology

Cite this

Role for serotonin3 receptors in the control of adrenocorticotropic hormone release from rat pituitary cell cultures. / Calogero, A. E.; Bagdy, G.; Burrello, N.; Polosa, P.; D'Agata, R.

In: European Journal of Endocrinology, Vol. 133, No. 2, 1995, p. 251-254.

Research output: Article

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AU - D'Agata, R.

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