Relationship of grafted FGF-2-overexpressing neural stem/progenitor cells with the vasculature in the cerebral cortex

Oleg Tsupykov, Michiko Kanemitsu, Ekaterina Smozhanik, Galina Skibo, Alexandre G. Dayer, J. Kiss

Research output: Article

3 Citations (Scopus)

Abstract

Neural progenitor cells (NPCs) overexpressing fibroblast growth factor 2 (FGF-2) have the distinct tendency to associate with the vasculature and establish multiple proliferative clusters in the perivascular environment after transplantation into the cerebral cortex. Strikingly, the vascular clusters of progenitor cells give rise to immature neurons after ischemic injury, raising prospects for the formation of ectopic neurogenic niches for repair. We investigated the spatial relationship of perivascular clusters with the host vascular structures. FGF-2-GFPtransduced NPCs were transplanted into the intact somatosensory rat cortex. Confocal microscopic analysis revealed that grafted cells preferentially contacted venules at sites with aquaporin-4-positive astrocytic endfeet and avoided contacts with desmin-positive pericytes. Electron microscopic analysis confirmed that grafted cells preferentially made contact with astroglial endfeet, and only a minority of them reached the endothelial basal lamina. These results provide new insights into the fine structural and anatomical relationship between grafted FGF-2-transduced NPCs and the host vasculature.

Original languageEnglish
Pages (from-to)1359-1369
Number of pages11
JournalCell Transplantation
Volume25
Issue number7
DOIs
Publication statusPublished - 2016

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Relationship of grafted FGF-2-overexpressing neural stem/progenitor cells with the vasculature in the cerebral cortex'. Together they form a unique fingerprint.

  • Cite this