Regulation of patulin-induced oxidative stress processes in the fission yeast Schizosaccharomyces pombe

Gábor Papp, Eszter Horváth, Nóra Mike, Zoltán Gazdag, József Belágyi, Zoltán Gyöngyi, Gáspár Bánfalvi, László Hornok, Miklós Pesti

Research output: Article

20 Citations (Scopus)


Patulin (PAT), is one of the most widely disseminated mycotoxins found in agricultural products. In this study the PAT-induced accumulation of reactive oxygen species (ROS) and the regulation of the specific activities of antioxidant enzymes were investigated in the single cell eukaryotic organism Schizosaccharomyces pombe. In comparison with the untreated cells, 500μM PAT treatment caused a 43% decrease in the concentration of the main intracellular antioxidant, glutathione (GSH); this depletion of GSH initiated a 2.44- and a 2.6-fold accumulation of superoxide anion and hydrogen peroxide, respectively, but did not increase the concentration of hydroxyl radicals; the reduction of ROS-induced adaptation processes via the activation of Pap1 transcription factor resulted in significantly increased specific activities of Cu/Zn superoxide dismutase, catalase and glutathione S-transferase to protect the cells against the ROS-induced unbalanced redox state. However, no change was measured in the activities of glutathione reductase, glutathione peroxidase and glucose-6-phosphate dehydrogenase. It seems reasonable to assume that the temporary PAT-induced ROS accumulation plays a crucial role in adaptation processes. The adverse effects of PAT may be exerted mainly through the destruction of cellular membranes and protein/enzyme functions.

Original languageEnglish
Pages (from-to)3792-3798
Number of pages7
JournalFood and Chemical Toxicology
Issue number10
Publication statusPublished - okt. 1 2012

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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