Regional differences in statistical geometry of endothelial dense granules in human extremity veins

G. Gősi, G. Nádasy, G. Raffai, E. Fehér, Z. Szeberin, E. Monos, G. Acsády

Research output: Article

1 Citation (Scopus)

Abstract

Objectives Leg and arm human veins are exposed to different gravitational stresses. We investigated if there is difference in the amount and geometry of secretory vesicles in their endothelium. Methods Superficial small vein segments were removed during vascular operations for electromicroscopic analysis. Vesicular area/total endothelial cross-sectional area was determined by computer-based morphometry. Long and short axes of granule cross sections were measured by image analyzing software. Results Vesicular density in all samples was 2.26 ± 0.34%. There was no significant difference between the vesicular densities of upper extremity and leg. The shape of the vesicles was more frequently elongated in leg than in arm sections (p 0.01). Conclusions The density of the vesicles does not depend on vascular region or orthostatic load. Ellipticity of these granules is significantly different in areas exposed to different gravitational stresses. This might contribute to the differences of thrombotic and hemodynamic properties of leg and upper body veins.

Original languageEnglish
Pages (from-to)481-485
Number of pages5
JournalPhlebology
Volume30
Issue number7
DOIs
Publication statusPublished - aug. 23 2015

Fingerprint

Veins
Leg
Extremities
Blood Vessels
Arm
Secretory Vesicles
Upper Extremity
Endothelium
Software
Hemodynamics

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Regional differences in statistical geometry of endothelial dense granules in human extremity veins. / Gősi, G.; Nádasy, G.; Raffai, G.; Fehér, E.; Szeberin, Z.; Monos, E.; Acsády, G.

In: Phlebology, Vol. 30, No. 7, 23.08.2015, p. 481-485.

Research output: Article

@article{996c8eea387941b8a45fd9ba9fabac9b,
title = "Regional differences in statistical geometry of endothelial dense granules in human extremity veins",
abstract = "Objectives Leg and arm human veins are exposed to different gravitational stresses. We investigated if there is difference in the amount and geometry of secretory vesicles in their endothelium. Methods Superficial small vein segments were removed during vascular operations for electromicroscopic analysis. Vesicular area/total endothelial cross-sectional area was determined by computer-based morphometry. Long and short axes of granule cross sections were measured by image analyzing software. Results Vesicular density in all samples was 2.26 ± 0.34{\%}. There was no significant difference between the vesicular densities of upper extremity and leg. The shape of the vesicles was more frequently elongated in leg than in arm sections (p 0.01). Conclusions The density of the vesicles does not depend on vascular region or orthostatic load. Ellipticity of these granules is significantly different in areas exposed to different gravitational stresses. This might contribute to the differences of thrombotic and hemodynamic properties of leg and upper body veins.",
keywords = "Endothelium, gravitation, morphometry, secretory vesicles, veins, Weibel–Palade bodies",
author = "G. Gősi and G. N{\'a}dasy and G. Raffai and E. Feh{\'e}r and Z. Szeberin and E. Monos and G. Acs{\'a}dy",
year = "2015",
month = "8",
day = "23",
doi = "10.1177/0268355514547715",
language = "English",
volume = "30",
pages = "481--485",
journal = "Phlebology",
issn = "1433-3031",
publisher = "SAGE Publications Ltd",
number = "7",

}

TY - JOUR

T1 - Regional differences in statistical geometry of endothelial dense granules in human extremity veins

AU - Gősi, G.

AU - Nádasy, G.

AU - Raffai, G.

AU - Fehér, E.

AU - Szeberin, Z.

AU - Monos, E.

AU - Acsády, G.

PY - 2015/8/23

Y1 - 2015/8/23

N2 - Objectives Leg and arm human veins are exposed to different gravitational stresses. We investigated if there is difference in the amount and geometry of secretory vesicles in their endothelium. Methods Superficial small vein segments were removed during vascular operations for electromicroscopic analysis. Vesicular area/total endothelial cross-sectional area was determined by computer-based morphometry. Long and short axes of granule cross sections were measured by image analyzing software. Results Vesicular density in all samples was 2.26 ± 0.34%. There was no significant difference between the vesicular densities of upper extremity and leg. The shape of the vesicles was more frequently elongated in leg than in arm sections (p 0.01). Conclusions The density of the vesicles does not depend on vascular region or orthostatic load. Ellipticity of these granules is significantly different in areas exposed to different gravitational stresses. This might contribute to the differences of thrombotic and hemodynamic properties of leg and upper body veins.

AB - Objectives Leg and arm human veins are exposed to different gravitational stresses. We investigated if there is difference in the amount and geometry of secretory vesicles in their endothelium. Methods Superficial small vein segments were removed during vascular operations for electromicroscopic analysis. Vesicular area/total endothelial cross-sectional area was determined by computer-based morphometry. Long and short axes of granule cross sections were measured by image analyzing software. Results Vesicular density in all samples was 2.26 ± 0.34%. There was no significant difference between the vesicular densities of upper extremity and leg. The shape of the vesicles was more frequently elongated in leg than in arm sections (p 0.01). Conclusions The density of the vesicles does not depend on vascular region or orthostatic load. Ellipticity of these granules is significantly different in areas exposed to different gravitational stresses. This might contribute to the differences of thrombotic and hemodynamic properties of leg and upper body veins.

KW - Endothelium

KW - gravitation

KW - morphometry

KW - secretory vesicles

KW - veins

KW - Weibel–Palade bodies

UR - http://www.scopus.com/inward/record.url?scp=84937809901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937809901&partnerID=8YFLogxK

U2 - 10.1177/0268355514547715

DO - 10.1177/0268355514547715

M3 - Article

VL - 30

SP - 481

EP - 485

JO - Phlebology

JF - Phlebology

SN - 1433-3031

IS - 7

ER -