Refolding of the human dihydrolipoamide dehydrogenase

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Abstract

A refolding procedure was optimized for human dihydrolipoamide dehydrogenase (LADH) that can serve as a complementary method to isolation protocols for cytosolic or periplasmic expression of the enzyme. The reported procedure is especially useful if part of the protein is precipitated into inclusion bodies during expression and higher quantities of the protein would be required without the need to increase the expression volume, like during protein labeling. Optimized parameters included the preparation/purification strategy of inclusion bodies, unfolding conditions, the composition of the refolding cocktail, time-dependence of the regained activity and dialysis conditions; other parameters were applied according to optimized values from previous studies or recommendations of the applied kits. The most efficient refolding mixture included 10 μM FAD and 10 mM DTT in 100 mM Tris, 150 mM NaCl, 1 mM EDTA at pH 8.0; this solution condition is also compatible to the subsequent Strep-tag affinity chromatography step applied for purification. The protein became fully active after refolding overnight. The final protein preparation showed a correct folding and a high purity owing to our optimized Strep-tag affinity purification strategy. The reported refolding protocol is able to generate up to 2.3 mg/L (of bacterial culture) active protein after complete purification.

Original languageEnglish
Pages (from-to)120-125
Number of pages6
JournalBiochemical Engineering Journal
Volume45
Issue number2
DOIs
Publication statusPublished - júl. 15 2009

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ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

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