Recovery of Proteins Affected by Mobile Phase Trifluoroacetic Acid Concentration in Reversed-Phase Chromatography

Balázs Bobály, Vivien Mikola, Enikő Sipkó, Zoltán Márta, J. Fekete

Research output: Article

3 Citations (Scopus)

Abstract

It was found that recoveries of proteins depend on trifluoroacetic acid concentration in the mobile phase and showed maximum in the range of 0.01-0.1 v/v%. Transferrin and lysozyme were used to evaluate the recoveries of proteins from dedicated reversed-phase columns. Different types of reversed-phase columns were evaluated, such as core shell type materials (Aeris Widepore with C4, C8 and C18 modification) as well as fully porous hybrid particles (Waters BEH, modified with C4 and C18 alkyl chains). Recoveries ranged between 60.7-95.2% for transferrin and 72.1-99.8% for lysozyme. Based on the data presented, at least two different adsorption effects, the well-known hydrophobic and silanophilic/polar interaction might influence the recovery. In addition to this, conformational effects due to ion pairing with the acidic mobile phase additive might change them.

Original languageEnglish
Pages (from-to)1078-1083
Number of pages6
JournalJournal of Chromatographic Science
Volume53
Issue number7
DOIs
Publication statusPublished - aug. 1 2015

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ASJC Scopus subject areas

  • Medicine(all)

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