For the production of thermostable endoglucanase from Clostridium thermocellulum the celC gene was cloned into Escherichia coli BL 21 (DE3) host. The recombinant E. coli was grown in shake flask cultures. The intracellular recombinant protein was extracted from the cells after applying supercritical CO2 cell disruption. The supercritical CO2 cell disintegration was optimized and then compared to the traditional ultrasonic cell disruption technique. With the supercritical cell disruption the cellulase recovery was approximately 17% lower than that of obtained with sonication.
|Number of pages||4|
|Journal||Chemical and Biochemical Engineering Quarterly|
|Publication status||Published - jún. 1 2003|
ASJC Scopus subject areas
- Process Chemistry and Technology