Rapid PCR based identification of two medically important dermatophyte fungi, microsporum canis and trichophyton tonsurans

Gabriela Malinovschi, Sándor Kocsubé, László Galgóczy, Ferenc Somogyvári, Csaba Vágvölgyi

Research output: Article

1 Citation (Scopus)

Abstract

PCR-based species specific protocols have been worked out for two important pathogens M. canis and T. tonsurans responsible for dermatophytosis with various clinical appearances. Reactions were designed to use a common reverse primer and 2 specific forward primers and were optimised to be efficient under the same PCR conditions allowing the detection of these two fungi from one reaction volume. The specific PCR reactions were evaluated both on collection strains of other dermatophytes and on clinical samples. In connection with these methods, different DNA extraction techniques were also tested to assist for an efficient PCR based detection.

Original languageEnglish
Pages (from-to)51-54
Number of pages4
JournalActa Biologica Szegediensis
Volume53
Issue number1
Publication statusPublished - dec. 1 2009

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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