Rapid and transient activation of a myelin basic protein kinase in tobacco leaves treated with harpin from Erwinia amylovora

A. Ádám, Sharon Pike, M. Elizabeth Hoyos, Julie M. Stone, John C. Walker, Anton Novacky

Research output: Article

76 Citations (Scopus)

Abstract

Harpins are bacterial protein elicitors that induce hypersensitive response-like necrosis when infiltrated into nonhost plants such as tobacco (Nicotiana tabacura L.) (Z.-M. Wei, R.J. Laby, C.H. Zumoff, D.W. Bauer, S.Y. He, A. Collmer, S.V. Beer [1992] Science 257: 85-88). Activity of a 49-kD Mg2+-dependent and Ca2+-independent kinase in tobacco leaves increased 50-fold 15 min after infiltration of harpin from Erwinia amylovora (harpin(Ea)). Much less pronounced and more transient activation was detected in water-infiltrated leaves. Biochemical characteristics of the harpin(Ea)-activated protein kinase (HAPK) activity are consistent with those of the mitogen-activated protein kinase family. HAPK is cytosolic and phosphorylates myelin basic protein on serine/threonine residues. Treatment with a protein tyrosine phosphatase completely eliminated HAPK activity, suggesting that tyrosine phosphorylation is required for posttranslational activation. Sustained HAPK activation after cycloheximide treatment implies that HAPK may be negatively regulated by a translation-dependent mechanism. The extracellular Ca2+ chelator EGTA or the protein kinase inhibitor K252a, infiltrated in planta together with harpin(Ea), partially blocked HAPK activation. The Ca2+-channel blocker La3+ had no effect on HAPK activation, suggesting that phosphorylation events precede and/or do not depend on the entry of extracellular Ca2+ into the cell. These results suggest that early signal transduction events during harpin(Ea)induced hypersensitive response elicitation depend in part on the activation of HAPK.

Original languageEnglish
Pages (from-to)853-861
Number of pages9
JournalPlant Physiology
Volume115
Issue number2
Publication statusPublished - 1997

Fingerprint

Erwinia amylovora
Myelin Basic Protein
protein kinases
Protein Kinases
Tobacco
tobacco
leaves
calcium
hypersensitive response
phosphorylation
Phosphorylation
Protein Tyrosine Phosphatases
Bacterial Proteins
protein-tyrosine-phosphatase
myelin basic protein
Egtazic Acid
bacterial proteins
Threonine
Cycloheximide
Protein Kinase Inhibitors

ASJC Scopus subject areas

  • Plant Science

Cite this

Rapid and transient activation of a myelin basic protein kinase in tobacco leaves treated with harpin from Erwinia amylovora. / Ádám, A.; Pike, Sharon; Hoyos, M. Elizabeth; Stone, Julie M.; Walker, John C.; Novacky, Anton.

In: Plant Physiology, Vol. 115, No. 2, 1997, p. 853-861.

Research output: Article

Ádám, A. ; Pike, Sharon ; Hoyos, M. Elizabeth ; Stone, Julie M. ; Walker, John C. ; Novacky, Anton. / Rapid and transient activation of a myelin basic protein kinase in tobacco leaves treated with harpin from Erwinia amylovora. In: Plant Physiology. 1997 ; Vol. 115, No. 2. pp. 853-861.
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