Raman-Based Feedback Control of the Enzymatic Hydrolysis of Lactose

Edit Hirsch, Hajnalka Pataki, Attila Farkas, Henrik Bata, Panna Vass, Csaba Fehér, Zsolt Barta, László Párta, István Csontos, András Ballagi, Zsombor K. Nagy, G. Marosi

Research output: Article

8 Citations (Scopus)

Abstract

The development of real-time monitoring and an improved control system was performed according to a process analytical technology initiative focusing on the benefits of the pharmaceutical industry. As an extension of the results of pioneering Raman-based closed-loop feedback control of crystallization and synthesis, a bioprocess, namely, the enzymatic hydrolysis of lactose, was investigated in this work. For this purpose, an effective control system was developed to maintain the lactose concentration at a level of 20 g/L. Real-time evaluation of Raman spectra was accomplished using classical least-squares (CLS) and partial least-squares (PLS) multivariate data analysis methods. Formation of oligosaccharides during the hydrolysis of lactose causes inaccuracy in the CLS evaluation, and thus, optimization of lactose hydrolysis was needed to decrease oligosaccharide formation. After optimization, control of lactose hydrolysis could be achieved. To improve real-time evaluation, the PLS method was used, resulting in better evaluation of spectra during control experiments.

Original languageEnglish
Pages (from-to)1721-1727
Number of pages7
JournalOrganic Process Research and Development
Volume20
Issue number10
DOIs
Publication statusPublished - okt. 21 2016

Fingerprint

lactose
Enzymatic hydrolysis
Lactose
feedback control
Feedback control
hydrolysis
Hydrolysis
evaluation
Oligosaccharides
Control systems
optimization
least squares method
Crystallization
Raman scattering
industries
crystallization
Raman spectra
Monitoring
causes
synthesis

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Raman-Based Feedback Control of the Enzymatic Hydrolysis of Lactose. / Hirsch, Edit; Pataki, Hajnalka; Farkas, Attila; Bata, Henrik; Vass, Panna; Fehér, Csaba; Barta, Zsolt; Párta, László; Csontos, István; Ballagi, András; Nagy, Zsombor K.; Marosi, G.

In: Organic Process Research and Development, Vol. 20, No. 10, 21.10.2016, p. 1721-1727.

Research output: Article

Hirsch, E, Pataki, H, Farkas, A, Bata, H, Vass, P, Fehér, C, Barta, Z, Párta, L, Csontos, I, Ballagi, A, Nagy, ZK & Marosi, G 2016, 'Raman-Based Feedback Control of the Enzymatic Hydrolysis of Lactose', Organic Process Research and Development, vol. 20, no. 10, pp. 1721-1727. https://doi.org/10.1021/acs.oprd.6b00212
Hirsch, Edit ; Pataki, Hajnalka ; Farkas, Attila ; Bata, Henrik ; Vass, Panna ; Fehér, Csaba ; Barta, Zsolt ; Párta, László ; Csontos, István ; Ballagi, András ; Nagy, Zsombor K. ; Marosi, G. / Raman-Based Feedback Control of the Enzymatic Hydrolysis of Lactose. In: Organic Process Research and Development. 2016 ; Vol. 20, No. 10. pp. 1721-1727.
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