Rab11 facilitates cross-talk between autophagy and endosomal pathway through regulation of Hook localization

Zsuzsanna Szatmári, Viktor Kis, Mónika Lippai, Krisztina Hegedus, Tamás Faragó, Péter Lorincz, Tsubasa Tanaka, Gábor Juhász, Miklós Sass

Research output: Article

59 Citations (Scopus)


During autophagy, double-membrane autophagosomes deliver sequestered cytoplasmic content to late endosomes and lysosomes for degradation. The molecular mechanism of autophagosome maturation is still poorly characterized. The small GTPase Rab11 regulates endosomal traffic and is thought to function at the level of recycling endosomes. We show that loss of Rab11 leads to accumulation of autophagosomes and late endosomes in Drosophila melanogaster. Rab11 translocates from recycling endosomes to autophagosomes in response to autophagy induction and physically interacts with Hook, a negative regulator of endosome maturation. Hook anchors endosomes to microtubules, and we show that Rab11 facilitates the fusion of endosomes and autophagosomes by removing Hook from mature late endosomes and inhibiting its homodimerization. Thus induction of autophagy appears to promote autophagic flux by increased convergence with the endosomal pathway.

Original languageEnglish
Pages (from-to)522-531
Number of pages10
JournalMolecular Biology of the Cell
Issue number4
Publication statusPublished - febr. 15 2014


ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this