Abstract
Background: The diagnosis of tick borne encephalitis (TBE) is mainly based on the demonstration of specific antibodies in serum when neurological disease is manifested. Improving diagnostics is the most important step in detecting and dealing with these pathogens. Quality control measures are essential for TBE diagnosis. Objective: To assess an external quality assurance (EQA) program for the serologic diagnosis of TBE infections. Study design: A panel of 12 serum samples was sent out to be tested for the presence of TBE virus-specific IgM and IgG. This panel contained seven TBE-positive samples for IgM and/or IgG; three negative samples; two samples positive either for West Nile virus (WNV) or Dengue virus (DENV). Results: Fourty-two laboratories from 25 European and 2 non-European countries participated in this EQA. The correct answer by each laboratory for all samples ranked between 58 and 96% and sera with IgM antibody positive for TBE were correctly recognized by 46-88% of the laboratories. Sera with IgG antibody positive for TBE were correctly recognized by 83-95% of the laboratories. False TBE-positive results were obtained with DENV, WNV or negative sera only for IgG-based assays. Conclusion: Correct results for at least 90% of the samples were obtained by 33 of 40 participating laboratories for IgM and for 16 of 42 laboratories for IgG.
| Original language | English |
|---|---|
| Pages (from-to) | 260-264 |
| Number of pages | 5 |
| Journal | Journal of Clinical Virology |
| Volume | 38 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - márc. 2007 |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Virology
- Immunology and Allergy
- Infectious Diseases
Cite this
Quality control assessment for the serological diagnosis of tick borne encephalitis virus infections. / Niedrig, Matthias; Avšič, Tatjana; Aberle, Stephan W.; Ferenczi, Emöke; Labuda, Milan; Rozentale, Baiba; Donoso Mantke, Oliver.
In: Journal of Clinical Virology, Vol. 38, No. 3, 03.2007, p. 260-264.Research output: Article
}
TY - JOUR
T1 - Quality control assessment for the serological diagnosis of tick borne encephalitis virus infections
AU - Niedrig, Matthias
AU - Avšič, Tatjana
AU - Aberle, Stephan W.
AU - Ferenczi, Emöke
AU - Labuda, Milan
AU - Rozentale, Baiba
AU - Donoso Mantke, Oliver
PY - 2007/3
Y1 - 2007/3
N2 - Background: The diagnosis of tick borne encephalitis (TBE) is mainly based on the demonstration of specific antibodies in serum when neurological disease is manifested. Improving diagnostics is the most important step in detecting and dealing with these pathogens. Quality control measures are essential for TBE diagnosis. Objective: To assess an external quality assurance (EQA) program for the serologic diagnosis of TBE infections. Study design: A panel of 12 serum samples was sent out to be tested for the presence of TBE virus-specific IgM and IgG. This panel contained seven TBE-positive samples for IgM and/or IgG; three negative samples; two samples positive either for West Nile virus (WNV) or Dengue virus (DENV). Results: Fourty-two laboratories from 25 European and 2 non-European countries participated in this EQA. The correct answer by each laboratory for all samples ranked between 58 and 96% and sera with IgM antibody positive for TBE were correctly recognized by 46-88% of the laboratories. Sera with IgG antibody positive for TBE were correctly recognized by 83-95% of the laboratories. False TBE-positive results were obtained with DENV, WNV or negative sera only for IgG-based assays. Conclusion: Correct results for at least 90% of the samples were obtained by 33 of 40 participating laboratories for IgM and for 16 of 42 laboratories for IgG.
AB - Background: The diagnosis of tick borne encephalitis (TBE) is mainly based on the demonstration of specific antibodies in serum when neurological disease is manifested. Improving diagnostics is the most important step in detecting and dealing with these pathogens. Quality control measures are essential for TBE diagnosis. Objective: To assess an external quality assurance (EQA) program for the serologic diagnosis of TBE infections. Study design: A panel of 12 serum samples was sent out to be tested for the presence of TBE virus-specific IgM and IgG. This panel contained seven TBE-positive samples for IgM and/or IgG; three negative samples; two samples positive either for West Nile virus (WNV) or Dengue virus (DENV). Results: Fourty-two laboratories from 25 European and 2 non-European countries participated in this EQA. The correct answer by each laboratory for all samples ranked between 58 and 96% and sera with IgM antibody positive for TBE were correctly recognized by 46-88% of the laboratories. Sera with IgG antibody positive for TBE were correctly recognized by 83-95% of the laboratories. False TBE-positive results were obtained with DENV, WNV or negative sera only for IgG-based assays. Conclusion: Correct results for at least 90% of the samples were obtained by 33 of 40 participating laboratories for IgM and for 16 of 42 laboratories for IgG.
KW - Arbovirus diagnostics
KW - External quality assurance: EQA
KW - Tick borne encephalitis
UR - http://www.scopus.com/inward/record.url?scp=33847047886&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847047886&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2006.12.013
DO - 10.1016/j.jcv.2006.12.013
M3 - Article
C2 - 17267281
AN - SCOPUS:33847047886
VL - 38
SP - 260
EP - 264
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
IS - 3
ER -