Pulmonary impact of titanium dioxide nanorods: Examination of nanorod-exposed rat lungs and human alveolar cells

Tamara Horváth, A. Papp, Nóra Igaz, Dávid Kovács, Gábor Kozma, Vivien Trenka, L. Tiszlavicz, Zsolt Rázga, Z. Kónya, Mónika Kiricsi, Tünde Vezér

Research output: Article

1 Citation (Scopus)

Abstract

Background: Titanium dioxide nanoparticles have numerous applications, resulting in human exposure. Nonetheless, available toxicological and safety data are insufficient regarding aspherical particles, such as rod-shaped nanoparticles. Methods: In a combined in vitro–in vivo approach, cultured A549 lung alveolar adenocar-cinoma cells were treated with approximately 15×65 nm TiO2 nanorod-containing medium, while young adult rats received the same substance by intratracheal instillation for 28 days in 5 and 18 mg/kg body-weight doses. Nanoparticle accumulation in the lungs and consequent oxidative stress, cell damage, and inflammation were assessed by biochemical and histopathological methods. Results: Titanium was detected in tissue samples by single-particle inductively coupled plasma mass spectrometry. Nanoparticles were visualized inside cultured A549 cells, within pulmonary macrophages, and in hilar lymph nodes of the rats. A549 cells showed dose-dependent oxidative stress and lethality, and the observed nanoparticle-laden endosomes suggested deranged lysosomal function and possible autophagy. Strongly elevated Ti levels were measured in the lungs of nanorod-treated rats and moderately elevated levels in the blood of the animals. Numerous cytokines, indicating acute and also chronic inflammation, were identified in the lung samples of TiO2-exposed rodents. Conclusion: Several signs of cell and tissue damage were detected in both the cultured alveolar cells and in treated rats’ lungs. Rod-shaped nanoparticulate TiO2 may consequently be more harmful than has generally been supposed. The occupational health risk suggested by the results calls for improved safety measures.

Original languageEnglish
Pages (from-to)7061-7077
Number of pages17
JournalInternational Journal of Nanomedicine
Volume13
DOIs
Publication statusPublished - jan. 1 2018

Fingerprint

Alveolar Epithelial Cells
Nanotubes
Nanorods
Nanoparticles
Titanium dioxide
Rats
Lung
Oxidative stress
Cultured Cells
Oxidative Stress
Occupational risks
Tissue
Inflammation
Safety
Inductively coupled plasma mass spectrometry
Health risks
Endosomes
Autophagy
Alveolar Macrophages
Occupational Health

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

Cite this

Pulmonary impact of titanium dioxide nanorods : Examination of nanorod-exposed rat lungs and human alveolar cells. / Horváth, Tamara; Papp, A.; Igaz, Nóra; Kovács, Dávid; Kozma, Gábor; Trenka, Vivien; Tiszlavicz, L.; Rázga, Zsolt; Kónya, Z.; Kiricsi, Mónika; Vezér, Tünde.

In: International Journal of Nanomedicine, Vol. 13, 01.01.2018, p. 7061-7077.

Research output: Article

Horváth, Tamara ; Papp, A. ; Igaz, Nóra ; Kovács, Dávid ; Kozma, Gábor ; Trenka, Vivien ; Tiszlavicz, L. ; Rázga, Zsolt ; Kónya, Z. ; Kiricsi, Mónika ; Vezér, Tünde. / Pulmonary impact of titanium dioxide nanorods : Examination of nanorod-exposed rat lungs and human alveolar cells. In: International Journal of Nanomedicine. 2018 ; Vol. 13. pp. 7061-7077.
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abstract = "Background: Titanium dioxide nanoparticles have numerous applications, resulting in human exposure. Nonetheless, available toxicological and safety data are insufficient regarding aspherical particles, such as rod-shaped nanoparticles. Methods: In a combined in vitro–in vivo approach, cultured A549 lung alveolar adenocar-cinoma cells were treated with approximately 15×65 nm TiO2 nanorod-containing medium, while young adult rats received the same substance by intratracheal instillation for 28 days in 5 and 18 mg/kg body-weight doses. Nanoparticle accumulation in the lungs and consequent oxidative stress, cell damage, and inflammation were assessed by biochemical and histopathological methods. Results: Titanium was detected in tissue samples by single-particle inductively coupled plasma mass spectrometry. Nanoparticles were visualized inside cultured A549 cells, within pulmonary macrophages, and in hilar lymph nodes of the rats. A549 cells showed dose-dependent oxidative stress and lethality, and the observed nanoparticle-laden endosomes suggested deranged lysosomal function and possible autophagy. Strongly elevated Ti levels were measured in the lungs of nanorod-treated rats and moderately elevated levels in the blood of the animals. Numerous cytokines, indicating acute and also chronic inflammation, were identified in the lung samples of TiO2-exposed rodents. Conclusion: Several signs of cell and tissue damage were detected in both the cultured alveolar cells and in treated rats’ lungs. Rod-shaped nanoparticulate TiO2 may consequently be more harmful than has generally been supposed. The occupational health risk suggested by the results calls for improved safety measures.",
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T1 - Pulmonary impact of titanium dioxide nanorods

T2 - Examination of nanorod-exposed rat lungs and human alveolar cells

AU - Horváth, Tamara

AU - Papp, A.

AU - Igaz, Nóra

AU - Kovács, Dávid

AU - Kozma, Gábor

AU - Trenka, Vivien

AU - Tiszlavicz, L.

AU - Rázga, Zsolt

AU - Kónya, Z.

AU - Kiricsi, Mónika

AU - Vezér, Tünde

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Titanium dioxide nanoparticles have numerous applications, resulting in human exposure. Nonetheless, available toxicological and safety data are insufficient regarding aspherical particles, such as rod-shaped nanoparticles. Methods: In a combined in vitro–in vivo approach, cultured A549 lung alveolar adenocar-cinoma cells were treated with approximately 15×65 nm TiO2 nanorod-containing medium, while young adult rats received the same substance by intratracheal instillation for 28 days in 5 and 18 mg/kg body-weight doses. Nanoparticle accumulation in the lungs and consequent oxidative stress, cell damage, and inflammation were assessed by biochemical and histopathological methods. Results: Titanium was detected in tissue samples by single-particle inductively coupled plasma mass spectrometry. Nanoparticles were visualized inside cultured A549 cells, within pulmonary macrophages, and in hilar lymph nodes of the rats. A549 cells showed dose-dependent oxidative stress and lethality, and the observed nanoparticle-laden endosomes suggested deranged lysosomal function and possible autophagy. Strongly elevated Ti levels were measured in the lungs of nanorod-treated rats and moderately elevated levels in the blood of the animals. Numerous cytokines, indicating acute and also chronic inflammation, were identified in the lung samples of TiO2-exposed rodents. Conclusion: Several signs of cell and tissue damage were detected in both the cultured alveolar cells and in treated rats’ lungs. Rod-shaped nanoparticulate TiO2 may consequently be more harmful than has generally been supposed. The occupational health risk suggested by the results calls for improved safety measures.

AB - Background: Titanium dioxide nanoparticles have numerous applications, resulting in human exposure. Nonetheless, available toxicological and safety data are insufficient regarding aspherical particles, such as rod-shaped nanoparticles. Methods: In a combined in vitro–in vivo approach, cultured A549 lung alveolar adenocar-cinoma cells were treated with approximately 15×65 nm TiO2 nanorod-containing medium, while young adult rats received the same substance by intratracheal instillation for 28 days in 5 and 18 mg/kg body-weight doses. Nanoparticle accumulation in the lungs and consequent oxidative stress, cell damage, and inflammation were assessed by biochemical and histopathological methods. Results: Titanium was detected in tissue samples by single-particle inductively coupled plasma mass spectrometry. Nanoparticles were visualized inside cultured A549 cells, within pulmonary macrophages, and in hilar lymph nodes of the rats. A549 cells showed dose-dependent oxidative stress and lethality, and the observed nanoparticle-laden endosomes suggested deranged lysosomal function and possible autophagy. Strongly elevated Ti levels were measured in the lungs of nanorod-treated rats and moderately elevated levels in the blood of the animals. Numerous cytokines, indicating acute and also chronic inflammation, were identified in the lung samples of TiO2-exposed rodents. Conclusion: Several signs of cell and tissue damage were detected in both the cultured alveolar cells and in treated rats’ lungs. Rod-shaped nanoparticulate TiO2 may consequently be more harmful than has generally been supposed. The occupational health risk suggested by the results calls for improved safety measures.

KW - Autophagy

KW - Cytokines

KW - Inflammation

KW - Nanoparticles

KW - Oxidative stress

KW - Toxicity

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