Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia

Daniel Martins-de-Souza, Wagner F. Gattaz, Andrea Schmitt, Giuseppina Maccarrone, E. Hunyadi-Gulyás, Marcos N. Eberlin, Gustavo H M F Souza, Sérgio Marangoni, José C. Novello, Christoph W. Turck, Emmanuel Dias-Neto

Research output: Article

116 Citations (Scopus)

Abstract

Schizophrenia is likely to be a consequence of serial alterations in a number of genes that, together with environmental factors, will lead to the establishment of the illness. The dorsolateral prefrontal cortex (Brodmann's Area 46) is implicated in schizophrenia and executes high functions such as working memory, differentiation of conflicting thoughts, determination of right and wrong concepts, correct social behavior and personality expression. We performed a comparative proteome analysis using two-dimensional gel electrophoresis of pools from 9 schizophrenia and 7 healthy control patients' dorsolateral prefrontal cortex aiming to identify, by mass spectrometry, alterations in protein expression that could be related to the disease. In schizophrenia-derived samples, our analysis revealed 10 downregulated and 14 upregulated proteins. These included alterations previously implicated in schizophrenia, such as oligodendrocyte-related proteins (myelin basic protein and transferrin), as well as malate dehydrogenase, aconitase, ATP synthase subunits and cytoskeleton-related proteins. Also, six new putative disease markers were identified, including energy metabolism, cytoskeleton and cell signaling proteins. Our data not only reinforces the involvement of proteins previously implicated in schizophrenia, but also suggests new markers, providing further information to foster the comprehension of this important disease.

Original languageEnglish
Pages (from-to)978-986
Number of pages9
JournalJournal of Psychiatric Research
Volume43
Issue number11
DOIs
Publication statusPublished - júl. 2009

Fingerprint

Oligodendroglia
Prefrontal Cortex
Cytoskeleton
Proteomics
Energy Metabolism
Schizophrenia
Proteins
Aconitate Hydratase
Malate Dehydrogenase
Myelin Basic Protein
Social Behavior
Electrophoresis, Gel, Two-Dimensional
Proteome
Transferrin
Short-Term Memory
Personality
Mass Spectrometry
Down-Regulation
Adenosine Triphosphate
Genes

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Biological Psychiatry

Cite this

Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia. / Martins-de-Souza, Daniel; Gattaz, Wagner F.; Schmitt, Andrea; Maccarrone, Giuseppina; Hunyadi-Gulyás, E.; Eberlin, Marcos N.; Souza, Gustavo H M F; Marangoni, Sérgio; Novello, José C.; Turck, Christoph W.; Dias-Neto, Emmanuel.

In: Journal of Psychiatric Research, Vol. 43, No. 11, 07.2009, p. 978-986.

Research output: Article

Martins-de-Souza, Daniel ; Gattaz, Wagner F. ; Schmitt, Andrea ; Maccarrone, Giuseppina ; Hunyadi-Gulyás, E. ; Eberlin, Marcos N. ; Souza, Gustavo H M F ; Marangoni, Sérgio ; Novello, José C. ; Turck, Christoph W. ; Dias-Neto, Emmanuel. / Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia. In: Journal of Psychiatric Research. 2009 ; Vol. 43, No. 11. pp. 978-986.
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