Propylene-glycol aggravates LPS-induced sepsis through production of TNF-α and IL-6

Annamaria Marton, Csongor Kolozsi, E. Kúsz, Zoltan Olah, Tamas Letoha, C. Vízler, Laszlo Pecze

Research output: Article

6 Citations (Scopus)

Abstract

Background: Propylene glycol (1,2-propanediol, PG) is a commonly used solvent for oral, intravenous, as well as topical pharmaceutical preparations. While PG is generally considered to be safe, it has been known that large intravenous doses given over a short period of time can be toxic. Objective: To evaluate the effect of PG in sepsis induced by the bacterial endotoxin lipopolysaccharide (LPS). Methods: Balb/c mice were treated with LPS (1 mg/kg b.w., i.p.) with or without PG (5 g/kg b.w. i.v.). The survival rate and the production of inflammatory cytokines were measured. In RAW264.7 mouse macrophages encoding NF-κB-luc reporter gene, the nuclear transcription factor kappa- B (NF-κB) activation was measured. Results: We found that intravenous PG increased the mortality rate in sepsis induced by the bacterial endotoxin lipopolysaccharide (LPS) in mice. In accordance with that, PG enhanced LPS-induced production of inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in vivo. PG also increased the LPS-induced macrophage activation in vitro as detected by measuring NF-κB activation. Conclusion: Our results indicate that drugs containing high doses of PG can pose a risk when administered to patients suffering from or prone to Gram negative bacterial infection.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalIranian Journal of Immunology
Volume11
Issue number2
Publication statusPublished - dec. 1 2014

Fingerprint

Propylene Glycol
Lipopolysaccharides
Interleukin-6
Sepsis
Tumor Necrosis Factor-alpha
NF-kappa B
Transcription Factors
Endotoxins
Gram-Negative Bacterial Infections
Cytokines
Macrophage Activation
Poisons
Reporter Genes
Pharmaceutical Preparations
Survival Rate
Macrophages
Mortality

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

Cite this

Propylene-glycol aggravates LPS-induced sepsis through production of TNF-α and IL-6. / Marton, Annamaria; Kolozsi, Csongor; Kúsz, E.; Olah, Zoltan; Letoha, Tamas; Vízler, C.; Pecze, Laszlo.

In: Iranian Journal of Immunology, Vol. 11, No. 2, 01.12.2014, p. 113-122.

Research output: Article

Marton, A, Kolozsi, C, Kúsz, E, Olah, Z, Letoha, T, Vízler, C & Pecze, L 2014, 'Propylene-glycol aggravates LPS-induced sepsis through production of TNF-α and IL-6', Iranian Journal of Immunology, vol. 11, no. 2, pp. 113-122.
Marton, Annamaria ; Kolozsi, Csongor ; Kúsz, E. ; Olah, Zoltan ; Letoha, Tamas ; Vízler, C. ; Pecze, Laszlo. / Propylene-glycol aggravates LPS-induced sepsis through production of TNF-α and IL-6. In: Iranian Journal of Immunology. 2014 ; Vol. 11, No. 2. pp. 113-122.
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