Projections from the vestibular nuclei to the hypothalamic paraventricular nucleus: Morphological evidence for the existence of a vestibular stress pathway in the rat brain

Balázs Markia, Z. Kovács, M. Palkóvits

Research output: Article

28 Citations (Scopus)

Abstract

Although it has been reported by several laboratories that vestibular stress activates the hypothalamo-pituitary-adrenocortical axis (HPA), the existence of neuronal connections between vestibular and hypothalamic paraventricular neurons has not yet been demonstrated. By the use of a virus-based retrograde trans-synaptic tracing technique in the rat, here we demonstrate vestibular projections to the paraventricular nucleus (PVN). Pseudorabies virus (Bartha strain, type BDR62) was injected into the PVN, and the progression of the infection along synaptically connected neurons was followed in the pons and the medulla, 3 and 4 days post-inoculation. Virus-infected neurons were revealed mainly in the medial vestibular nucleus. Labeled cells were scattered in the spinal, and very rarely in the superior nuclei, but none of them in the lateral vestibular nucleus. Injections of cholera toxin B subunit, a monosynaptic retrograde tracer into the PVN failed to label any cells in the vestibular nuclei. These results provide anatomical evidence for the existence of a vestibulo-paraventricular polysynaptic pathway and support the view that the HPA axis is modulated by vestibular stress.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalBrain Structure and Function
Volume213
Issue number1-2
DOIs
Publication statusPublished - szept. 2008

Fingerprint

Vestibular Nuclei
Paraventricular Hypothalamic Nucleus
Neurons
Brain
Lateral Vestibular Nucleus
Viruses
Suid Herpesvirus 1
Pons
Cholera Toxin
Injections
Infection

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Neuroscience(all)

Cite this

@article{4792ec387c914b70aca71a60d79b699f,
title = "Projections from the vestibular nuclei to the hypothalamic paraventricular nucleus: Morphological evidence for the existence of a vestibular stress pathway in the rat brain",
abstract = "Although it has been reported by several laboratories that vestibular stress activates the hypothalamo-pituitary-adrenocortical axis (HPA), the existence of neuronal connections between vestibular and hypothalamic paraventricular neurons has not yet been demonstrated. By the use of a virus-based retrograde trans-synaptic tracing technique in the rat, here we demonstrate vestibular projections to the paraventricular nucleus (PVN). Pseudorabies virus (Bartha strain, type BDR62) was injected into the PVN, and the progression of the infection along synaptically connected neurons was followed in the pons and the medulla, 3 and 4 days post-inoculation. Virus-infected neurons were revealed mainly in the medial vestibular nucleus. Labeled cells were scattered in the spinal, and very rarely in the superior nuclei, but none of them in the lateral vestibular nucleus. Injections of cholera toxin B subunit, a monosynaptic retrograde tracer into the PVN failed to label any cells in the vestibular nuclei. These results provide anatomical evidence for the existence of a vestibulo-paraventricular polysynaptic pathway and support the view that the HPA axis is modulated by vestibular stress.",
keywords = "Paraventricular nucleus, Retrograde tract-tracing, Trans-synaptic viral labeling, Vestibular nuclei, Vestibulo-hypothalamic connections",
author = "Bal{\'a}zs Markia and Z. Kov{\'a}cs and M. Palk{\'o}vits",
year = "2008",
month = "9",
doi = "10.1007/s00429-008-0172-6",
language = "English",
volume = "213",
pages = "239--245",
journal = "Referate und Beiträge zur Anatomie und Entwickelungsgeschichte",
issn = "0177-5154",
publisher = "Springer Verlag",
number = "1-2",

}

TY - JOUR

T1 - Projections from the vestibular nuclei to the hypothalamic paraventricular nucleus

T2 - Morphological evidence for the existence of a vestibular stress pathway in the rat brain

AU - Markia, Balázs

AU - Kovács, Z.

AU - Palkóvits, M.

PY - 2008/9

Y1 - 2008/9

N2 - Although it has been reported by several laboratories that vestibular stress activates the hypothalamo-pituitary-adrenocortical axis (HPA), the existence of neuronal connections between vestibular and hypothalamic paraventricular neurons has not yet been demonstrated. By the use of a virus-based retrograde trans-synaptic tracing technique in the rat, here we demonstrate vestibular projections to the paraventricular nucleus (PVN). Pseudorabies virus (Bartha strain, type BDR62) was injected into the PVN, and the progression of the infection along synaptically connected neurons was followed in the pons and the medulla, 3 and 4 days post-inoculation. Virus-infected neurons were revealed mainly in the medial vestibular nucleus. Labeled cells were scattered in the spinal, and very rarely in the superior nuclei, but none of them in the lateral vestibular nucleus. Injections of cholera toxin B subunit, a monosynaptic retrograde tracer into the PVN failed to label any cells in the vestibular nuclei. These results provide anatomical evidence for the existence of a vestibulo-paraventricular polysynaptic pathway and support the view that the HPA axis is modulated by vestibular stress.

AB - Although it has been reported by several laboratories that vestibular stress activates the hypothalamo-pituitary-adrenocortical axis (HPA), the existence of neuronal connections between vestibular and hypothalamic paraventricular neurons has not yet been demonstrated. By the use of a virus-based retrograde trans-synaptic tracing technique in the rat, here we demonstrate vestibular projections to the paraventricular nucleus (PVN). Pseudorabies virus (Bartha strain, type BDR62) was injected into the PVN, and the progression of the infection along synaptically connected neurons was followed in the pons and the medulla, 3 and 4 days post-inoculation. Virus-infected neurons were revealed mainly in the medial vestibular nucleus. Labeled cells were scattered in the spinal, and very rarely in the superior nuclei, but none of them in the lateral vestibular nucleus. Injections of cholera toxin B subunit, a monosynaptic retrograde tracer into the PVN failed to label any cells in the vestibular nuclei. These results provide anatomical evidence for the existence of a vestibulo-paraventricular polysynaptic pathway and support the view that the HPA axis is modulated by vestibular stress.

KW - Paraventricular nucleus

KW - Retrograde tract-tracing

KW - Trans-synaptic viral labeling

KW - Vestibular nuclei

KW - Vestibulo-hypothalamic connections

UR - http://www.scopus.com/inward/record.url?scp=50249149544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50249149544&partnerID=8YFLogxK

U2 - 10.1007/s00429-008-0172-6

DO - 10.1007/s00429-008-0172-6

M3 - Article

C2 - 18247051

AN - SCOPUS:50249149544

VL - 213

SP - 239

EP - 245

JO - Referate und Beiträge zur Anatomie und Entwickelungsgeschichte

JF - Referate und Beiträge zur Anatomie und Entwickelungsgeschichte

SN - 0177-5154

IS - 1-2

ER -