Programmed cell death in Datura innoxia Mill. callus cultures cultivated in light and in dark

I. László, É Szke, E. Tyihák, B. Szende

Research output: Article

2 Citations (Scopus)

Abstract

It has been established that dimedone in solid culture medium influenced the growth of Datura innoxia Mill. callus tissues and the apoptotic processes of cells. This formaldehyde (HCHO) capture reagent appears to modify the metabolism of plant cells, resulting in quantitative changes in the apoptotic index (Ai). Apoptotic cells were detected in six-week-old callus tissues by the TUNEL reaction. The amount of TUNEL positive nuclei showed a characteristic spatial distribution. Enhanced DNA fragmentation was observed in the cell layers close to the surface of the cultures. Elevated Ai was determined in cultures grown in the dark compared to the tissues grown in the light. High doses of dimedone considerably decreased apoptosis in tissue cultures under both light and dark conditions.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalPlant Growth Regulation
Volume33
Issue number3
DOIs
Publication statusPublished - okt. 9 2001

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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