Production and purification of low calcium response protein H of Chlamydophila pneumoniae

Ildikó Faludi, Ágnes Csanádi, Ágnes Míra Szabó, Katalin Burián, Valéria Endrész, A. Miczák

Research output: Article

1 Citation (Scopus)

Abstract

Chlamydophila pneumoniae possesses a type III secretion system (TTSS), which allows the bacteria to secrete effector molecules into the inclusion membrane and into the cytosol of the host cell. Low calcium response protein H (LcrH), as a part of the TTSS, is a chaperone protein expressed from the middle to late stages of the chlamydial developmental cycle. Gene of LcrH (CPn0811) in a 6His-tagged form was cloned from C. pneumoniae CWL029, expressed and purified from Escherichia coli using the HIS-select TALON CellThru Resin. The purity was checked with mass spectrometry. The samples were used for immunization of BALB/c mice. The inducible E. coli clone, which over-expresses the chlamydial LcrH, permits the study of the biological properties of this protein.

Original languageEnglish
Pages (from-to)389-397
Number of pages9
JournalActa microbiologica et immunologica Hungarica
Volume56
Issue number4
DOIs
Publication statusPublished - dec. 1 2009

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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