Preparative scale purification of shidasterone, 2-deoxy-polypodine B and 9α,20-dihydroxyecdysone from Silene italica ssp. nemoralis

Mária Báthori, Zita Pongrácz, Róbert Omacht, Imre Máthé

Research output: Article

3 Citations (Scopus)


A suitable combination of preparative scale separation methods results in effective clean-up of the ecdysteroids of Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The isolation of minor ecdysteroids from the partially purified extract is based on the use of both droplet counter-current chromatography and low-pressure reversed-phase liquid chromatography. The purification is completed by preparative thin-layer chromatography and preparative high-performance liquid chromatography to obtain the minor ecdysteroids, such as 2-deoxy-20-hydroxyecdysone, shidasterone, 2-deoxy-polypodine B, makisterone C, and 9α,20-dihydroxyecdysone.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalJournal of chromatographic science
Issue number5
Publication statusPublished - jan. 1 2004


ASJC Scopus subject areas

  • Analytical Chemistry

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