Porous silicon/photosynthetic reaction center hybrid nanostructure

Kata Hajdu, Csilla Gergely, Marta Martin, Thierry Cloitre, László Zimányi, Katalin Tenger, Petro Khoroshyy, Gabriela Palestino, Vivechana Agarwal, Klára Hernádi, Zoltán Németh, László Nagy

Research output: Article

22 Citations (Scopus)

Abstract

The purified photosynthetic reaction center protein (RC) from Rhodobacter sphaeroides R-26 purple bacteria was bound to porous silicon microcavities (PSiMc) either through silane-glutaraldehyde (GTA) chemistry or via a noncovalent peptide cross-linker. The characteristic resonance mode in the microcavity reflectivity spectrum red shifted by several nanometers upon RC binding, indicating the protein infiltration into the porous silicon (PSi) photonic structure. Flash photolysis experiments confirmed the photochemical activity of RC after its binding to the solid substrate. The kinetic components of the intraprotein charge recombination were considerably faster (τfast = 14 (±9) ms, τslow = 230 (±28) ms with the RC bound through the GTA cross-linker and only τfast = 27 (±3) ms through peptide coating) than in solution (τfast = 120 (±3) ms, τslow = 1387 (±2) ms), indicating the effect of the PSi surface on the light-induced electron transfer in the protein. The PSi/RC complex was found to oxidize the externally added electron donor, mammalian cytochrome c, and the cytochrome oxidation was blocked by the competitive RC inhibitor, terbutryne. This fact indicates that the specific surface binding sites on the PSi-bound RC are still accessible to external cofactors and an electronic interaction with redox components in the aqueous environment is possible. This new type of biophotonic material is considered to be an excellent model for new generation applications at the interface of silicon-based electronics and biological redox systems designed by nature.

Original languageEnglish
Pages (from-to)11866-11873
Number of pages8
JournalLangmuir
Volume28
Issue number32
DOIs
Publication statusPublished - aug. 14 2012

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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  • Cite this

    Hajdu, K., Gergely, C., Martin, M., Cloitre, T., Zimányi, L., Tenger, K., Khoroshyy, P., Palestino, G., Agarwal, V., Hernádi, K., Németh, Z., & Nagy, L. (2012). Porous silicon/photosynthetic reaction center hybrid nanostructure. Langmuir, 28(32), 11866-11873. https://doi.org/10.1021/la301888p