Plasmalemma-bound ATPase of potato tubers and its inhibition by Phytophthora infestans-derived elicitors of the hypersensitive reaction

E. P. Ladyzhenskaya, A. Ádám, N. P. Korableva, T. Érsek

Research output: Article

17 Citations (Scopus)

Abstract

Plasmalemma (PM) vesicles were isolated from tuber disks of potato cv. Kennebec and ATPase activity was characterized. The activity was synergistically stimulated by K+ and Na+ in the presence of Mg2+ at a pH optimum of 6.5 and increased by the canalogenic antibiotic, alamethicin, suggesting that the enzyme is located inside the right side-out vesicles. It was, on the other hand, inhibited by diethylstilbestrol, ouabain and orthovanadate. Arachidonic acid (AA), present in potato late blight fungus, Phytophthora infestans and considered as a pure elicitor of the hypersensitive resistance response of potato, markedly decreased the (K+ + Na+)-stimulated activity of the ATPase when added to PM preparation. The mycelial homogenate, which has been termed a crude elicitor and which also contains AA, had a negligible effect on the enzyme. Both crude elicitor and AA inhibited the ATPase activity when applied to tuber disks. The inhibition was expressed within 3 h after treatments as a result of one of the earliest actions of elicitors. The effect on the ATPase might be the cause of other metabolic changes that finally manifest themselves in the hypersensitive reaction.

Original languageEnglish
Pages (from-to)137-142
Number of pages6
JournalPlant Science
Volume73
Issue number2
DOIs
Publication statusPublished - 1991

Fingerprint

Phytophthora infestans
hypersensitive response
Solanum tuberosum
adenosinetriphosphatase
Adenosine Triphosphatases
tubers
plasma membrane
arachidonic acid
Arachidonic Acid
potatoes
alamethicin
Alamethicin
diethylstilbestrol
ouabain
Diethylstilbestrol
Vanadates
Ouabain
Enzymes
enzymes
Fungi

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Plasmalemma-bound ATPase of potato tubers and its inhibition by Phytophthora infestans-derived elicitors of the hypersensitive reaction. / Ladyzhenskaya, E. P.; Ádám, A.; Korableva, N. P.; Érsek, T.

In: Plant Science, Vol. 73, No. 2, 1991, p. 137-142.

Research output: Article

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AU - Érsek, T.

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