Plant polysaccharide degrading enzyme system of Thermobifida cellulosilytica TB100T revealed by de novo genome project data

Tóth, E. Baka, Sz Luzics, I. Bata-Vidács, I. Nagy, B. Bálint, R. Herczeg, F. Olasz, T. Wilk, T. Nagy, B. Kriszt, I. Nagy, J. Kukolya

Research output: Article

Abstract

Thermobifidas are thermophilic, aerobic, lignocellulose decomposing actinomycetes. The Thermobifida genus includes four species: T. fusca, T. alba, T. cellulosilytica, and T. halotolerans. T. fusca YX is the far best characterized strain of this taxon and several cellulases and hemicellulases have been cloned from it for industrial purposes targeting paper industry, biofuel, and feed applications. Unfortunately, sequence data of such enzymes are almost exclusively restricted to this single species; however, we demonstrated earlier by zymography that other T. alba and T. cellulosilytica strains encode the same enzyme sets. Recently, the advances in whole genome sequencing by the use of next generation genomics platforms accelerated the selection process of valuable hydrolases from uncharacterized bacterial species for cloning purposes. For this purpose T. cellulosilytica TB100T type strain was chosen for de novo genome sequencing. We have assembled the genome of T. cellulosilytica strain TB100T into 168 contigs and 19 scaffolds, with reference length of 4 327 869 bps, 3 589 putative coding sequences, 53 tRNAs, and 4 rRNAs. The analysis of the annotated genome revealed the existence of 27 putative hydrolases belonging to 14 different glycoside hydrolase (GH) families. The investigation of identified, cloned, and heterologously multiple cellulases, mannanases, xylanases, and amylases may result in industrial applications beside gaining useful basic research related information.

Original languageEnglish
Pages (from-to)323-335
Number of pages13
JournalActa Alimentaria
Volume46
Issue number3
DOIs
Publication statusPublished - szept. 1 2017

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Thermobifida
Polysaccharides
Thermobifida fusca
polysaccharides
hydrolases
Genome
Cellulases
genome
cellulases
Hydrolases
Enzymes
enzymes
Thermobifida alba
beta-mannosidase
lignocellulose
pulp and paper industry
Biofuels
Actinobacteria
Glycoside Hydrolases
industrial applications

ASJC Scopus subject areas

  • Food Science

Cite this

Plant polysaccharide degrading enzyme system of Thermobifida cellulosilytica TB100T revealed by de novo genome project data. / Tóth; Baka, E.; Luzics, Sz; Bata-Vidács, I.; Nagy, I.; Bálint, B.; Herczeg, R.; Olasz, F.; Wilk, T.; Nagy, T.; Kriszt, B.; Nagy, I.; Kukolya, J.

In: Acta Alimentaria, Vol. 46, No. 3, 01.09.2017, p. 323-335.

Research output: Article

Tóth, Baka, E, Luzics, S, Bata-Vidács, I, Nagy, I, Bálint, B, Herczeg, R, Olasz, F, Wilk, T, Nagy, T, Kriszt, B, Nagy, I & Kukolya, J 2017, 'Plant polysaccharide degrading enzyme system of Thermobifida cellulosilytica TB100T revealed by de novo genome project data', Acta Alimentaria, vol. 46, no. 3, pp. 323-335. https://doi.org/10.1556/066.2016.0014
Tóth ; Baka, E. ; Luzics, Sz ; Bata-Vidács, I. ; Nagy, I. ; Bálint, B. ; Herczeg, R. ; Olasz, F. ; Wilk, T. ; Nagy, T. ; Kriszt, B. ; Nagy, I. ; Kukolya, J. / Plant polysaccharide degrading enzyme system of Thermobifida cellulosilytica TB100T revealed by de novo genome project data. In: Acta Alimentaria. 2017 ; Vol. 46, No. 3. pp. 323-335.
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AU - Bata-Vidács, I.

AU - Nagy, I.

AU - Bálint, B.

AU - Herczeg, R.

AU - Olasz, F.

AU - Wilk, T.

AU - Nagy, T.

AU - Kriszt, B.

AU - Nagy, I.

AU - Kukolya, J.

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N2 - Thermobifidas are thermophilic, aerobic, lignocellulose decomposing actinomycetes. The Thermobifida genus includes four species: T. fusca, T. alba, T. cellulosilytica, and T. halotolerans. T. fusca YX is the far best characterized strain of this taxon and several cellulases and hemicellulases have been cloned from it for industrial purposes targeting paper industry, biofuel, and feed applications. Unfortunately, sequence data of such enzymes are almost exclusively restricted to this single species; however, we demonstrated earlier by zymography that other T. alba and T. cellulosilytica strains encode the same enzyme sets. Recently, the advances in whole genome sequencing by the use of next generation genomics platforms accelerated the selection process of valuable hydrolases from uncharacterized bacterial species for cloning purposes. For this purpose T. cellulosilytica TB100T type strain was chosen for de novo genome sequencing. We have assembled the genome of T. cellulosilytica strain TB100T into 168 contigs and 19 scaffolds, with reference length of 4 327 869 bps, 3 589 putative coding sequences, 53 tRNAs, and 4 rRNAs. The analysis of the annotated genome revealed the existence of 27 putative hydrolases belonging to 14 different glycoside hydrolase (GH) families. The investigation of identified, cloned, and heterologously multiple cellulases, mannanases, xylanases, and amylases may result in industrial applications beside gaining useful basic research related information.

AB - Thermobifidas are thermophilic, aerobic, lignocellulose decomposing actinomycetes. The Thermobifida genus includes four species: T. fusca, T. alba, T. cellulosilytica, and T. halotolerans. T. fusca YX is the far best characterized strain of this taxon and several cellulases and hemicellulases have been cloned from it for industrial purposes targeting paper industry, biofuel, and feed applications. Unfortunately, sequence data of such enzymes are almost exclusively restricted to this single species; however, we demonstrated earlier by zymography that other T. alba and T. cellulosilytica strains encode the same enzyme sets. Recently, the advances in whole genome sequencing by the use of next generation genomics platforms accelerated the selection process of valuable hydrolases from uncharacterized bacterial species for cloning purposes. For this purpose T. cellulosilytica TB100T type strain was chosen for de novo genome sequencing. We have assembled the genome of T. cellulosilytica strain TB100T into 168 contigs and 19 scaffolds, with reference length of 4 327 869 bps, 3 589 putative coding sequences, 53 tRNAs, and 4 rRNAs. The analysis of the annotated genome revealed the existence of 27 putative hydrolases belonging to 14 different glycoside hydrolase (GH) families. The investigation of identified, cloned, and heterologously multiple cellulases, mannanases, xylanases, and amylases may result in industrial applications beside gaining useful basic research related information.

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KW - Glycoside hydrolyses

KW - Hemicellulases

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