Placing Eophila tellinii (Oligochaeta, Lumbricidae) in a molecular phylogenetic context advances the century-old controversy around the problematic genus

Irene de Sosa, Darío J. Díaz Cosín, C. Csuzdi, Maurizzio G. Paoletti, Daniel F. Marchán

Research output: Article

Abstract

The genus Eophila Rosa, 1893 has been one of the most controversial in the taxonomy of Lumbricidae. The most accomplished earthworm experts from the last century accepted, rejected or split it into different genera depending on the studied species or morphological characters. In order to delimit a monophyletic, evolutionary significant Eophila, its type species Eophila tellinii was sequenced for mitochondrial (COI, 16S, tRNAs) and nuclear (28S) markers and included in a molecular phylogenetics context. Its putative close relative Eophila gestroi was also included in the same way, like Pietromodeona janueargenti, an enigmatic Tyrrhenian lumbricid. Bayesian inference and divergence time estimation were used to find the phylogenetic placement of the aforementioned species and to compare the age of the different genus-level clades of Lumbricidae. Eophila tellinii was recovered in a well-supported clade together with Eophila gestroi and Eophila crodabepis, clearly separated from other species previously assigned to the genus. In addition, this clade was found to be of a similar age (around 44 mya) to other studied genera and genus-level clades. The ultrametric tree provided preliminary insight on the paleobiogeography and diversification of the family. Relevant taxa to include in further molecular phylogenetic studies and the necessity of an integrative systematic revision are highlighted by the conclusions of this work.

Original languageEnglish
Article number103114
JournalEuropean Journal of Soil Biology
Volume94
DOIs
Publication statusPublished - szept. 1 2019

Fingerprint

Mya
Lumbricidae
Oligochaeta
Transfer RNA
phylogenetics
phylogeny
earthworms
paleobiogeography
Rosa
earthworm
taxonomy
divergence

ASJC Scopus subject areas

  • Microbiology
  • Soil Science
  • Insect Science

Cite this

Placing Eophila tellinii (Oligochaeta, Lumbricidae) in a molecular phylogenetic context advances the century-old controversy around the problematic genus. / de Sosa, Irene; Díaz Cosín, Darío J.; Csuzdi, C.; Paoletti, Maurizzio G.; Marchán, Daniel F.

In: European Journal of Soil Biology, Vol. 94, 103114, 01.09.2019.

Research output: Article

@article{67bce60d166946feb7568be9146da55b,
title = "Placing Eophila tellinii (Oligochaeta, Lumbricidae) in a molecular phylogenetic context advances the century-old controversy around the problematic genus",
abstract = "The genus Eophila Rosa, 1893 has been one of the most controversial in the taxonomy of Lumbricidae. The most accomplished earthworm experts from the last century accepted, rejected or split it into different genera depending on the studied species or morphological characters. In order to delimit a monophyletic, evolutionary significant Eophila, its type species Eophila tellinii was sequenced for mitochondrial (COI, 16S, tRNAs) and nuclear (28S) markers and included in a molecular phylogenetics context. Its putative close relative Eophila gestroi was also included in the same way, like Pietromodeona janueargenti, an enigmatic Tyrrhenian lumbricid. Bayesian inference and divergence time estimation were used to find the phylogenetic placement of the aforementioned species and to compare the age of the different genus-level clades of Lumbricidae. Eophila tellinii was recovered in a well-supported clade together with Eophila gestroi and Eophila crodabepis, clearly separated from other species previously assigned to the genus. In addition, this clade was found to be of a similar age (around 44 mya) to other studied genera and genus-level clades. The ultrametric tree provided preliminary insight on the paleobiogeography and diversification of the family. Relevant taxa to include in further molecular phylogenetic studies and the necessity of an integrative systematic revision are highlighted by the conclusions of this work.",
keywords = "Bayesian inference, Divergence time estimation, Earthworms, Systematics",
author = "{de Sosa}, Irene and {D{\'i}az Cos{\'i}n}, {Dar{\'i}o J.} and C. Csuzdi and Paoletti, {Maurizzio G.} and March{\'a}n, {Daniel F.}",
year = "2019",
month = "9",
day = "1",
doi = "10.1016/j.ejsobi.2019.103114",
language = "English",
volume = "94",
journal = "European Journal of Soil Biology",
issn = "1164-5563",
publisher = "Elsevier Masson SAS",

}

TY - JOUR

T1 - Placing Eophila tellinii (Oligochaeta, Lumbricidae) in a molecular phylogenetic context advances the century-old controversy around the problematic genus

AU - de Sosa, Irene

AU - Díaz Cosín, Darío J.

AU - Csuzdi, C.

AU - Paoletti, Maurizzio G.

AU - Marchán, Daniel F.

PY - 2019/9/1

Y1 - 2019/9/1

N2 - The genus Eophila Rosa, 1893 has been one of the most controversial in the taxonomy of Lumbricidae. The most accomplished earthworm experts from the last century accepted, rejected or split it into different genera depending on the studied species or morphological characters. In order to delimit a monophyletic, evolutionary significant Eophila, its type species Eophila tellinii was sequenced for mitochondrial (COI, 16S, tRNAs) and nuclear (28S) markers and included in a molecular phylogenetics context. Its putative close relative Eophila gestroi was also included in the same way, like Pietromodeona janueargenti, an enigmatic Tyrrhenian lumbricid. Bayesian inference and divergence time estimation were used to find the phylogenetic placement of the aforementioned species and to compare the age of the different genus-level clades of Lumbricidae. Eophila tellinii was recovered in a well-supported clade together with Eophila gestroi and Eophila crodabepis, clearly separated from other species previously assigned to the genus. In addition, this clade was found to be of a similar age (around 44 mya) to other studied genera and genus-level clades. The ultrametric tree provided preliminary insight on the paleobiogeography and diversification of the family. Relevant taxa to include in further molecular phylogenetic studies and the necessity of an integrative systematic revision are highlighted by the conclusions of this work.

AB - The genus Eophila Rosa, 1893 has been one of the most controversial in the taxonomy of Lumbricidae. The most accomplished earthworm experts from the last century accepted, rejected or split it into different genera depending on the studied species or morphological characters. In order to delimit a monophyletic, evolutionary significant Eophila, its type species Eophila tellinii was sequenced for mitochondrial (COI, 16S, tRNAs) and nuclear (28S) markers and included in a molecular phylogenetics context. Its putative close relative Eophila gestroi was also included in the same way, like Pietromodeona janueargenti, an enigmatic Tyrrhenian lumbricid. Bayesian inference and divergence time estimation were used to find the phylogenetic placement of the aforementioned species and to compare the age of the different genus-level clades of Lumbricidae. Eophila tellinii was recovered in a well-supported clade together with Eophila gestroi and Eophila crodabepis, clearly separated from other species previously assigned to the genus. In addition, this clade was found to be of a similar age (around 44 mya) to other studied genera and genus-level clades. The ultrametric tree provided preliminary insight on the paleobiogeography and diversification of the family. Relevant taxa to include in further molecular phylogenetic studies and the necessity of an integrative systematic revision are highlighted by the conclusions of this work.

KW - Bayesian inference

KW - Divergence time estimation

KW - Earthworms

KW - Systematics

UR - http://www.scopus.com/inward/record.url?scp=85069726025&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85069726025&partnerID=8YFLogxK

U2 - 10.1016/j.ejsobi.2019.103114

DO - 10.1016/j.ejsobi.2019.103114

M3 - Article

VL - 94

JO - European Journal of Soil Biology

JF - European Journal of Soil Biology

SN - 1164-5563

M1 - 103114

ER -