Placenta-specific gene manipulation in rabbits

Gabriella Skoda, Orsolya Ivett Hoffmann, Elen Gócza, Lilla Bodrogi, Andrea Kerekes, Z. Bösze, Laszlo Hiripi

Research output: Article

3 Citations (Scopus)

Abstract

Lentiviral gene constructs can be efficiently and specifically delivered to trophoblast cell lineages in rodents. In vivo genetic manipulation of trophoblast cell lines enables functional and developmental studies in the placenta. In this report we show that genetic modification can be produced in the extraembryonic tissues of rabbits by lentiviral gene constructs. When 8–16 cell stage embryos were injected with lentiviral particles, strong reporter gene expression resulted in the rabbit placenta. The expression pattern displayed some mosaicism. A strikingly high degree of mosaic GFP expression was detected in some parts of the yolk sac, which is a hypoblast-derived tissue. Whereas expression of the reporter gene construct was detected in placentas and yolk sacs, fetuses never expressed the transgene. As rabbits are an ideal model for functional studies in the placenta, our method would open new possibilities in rabbit biotechnology and placentation studies.

Original languageEnglish
Pages (from-to)86-90
Number of pages5
JournalJournal of Biotechnology
Volume259
DOIs
Publication statusPublished - okt. 10 2017

Fingerprint

Placenta
Genes
Rabbits
Yolk Sac
Trophoblasts
Reporter Genes
Tissue
Biotechnology
Gene expression
Germ Layers
Placentation
Mosaicism
Cell Lineage
Transgenes
Cells
Rodentia
Fetus
Embryonic Structures
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Skoda, G., Hoffmann, O. I., Gócza, E., Bodrogi, L., Kerekes, A., Bösze, Z., & Hiripi, L. (2017). Placenta-specific gene manipulation in rabbits. Journal of Biotechnology, 259, 86-90. https://doi.org/10.1016/j.jbiotec.2017.07.037

Placenta-specific gene manipulation in rabbits. / Skoda, Gabriella; Hoffmann, Orsolya Ivett; Gócza, Elen; Bodrogi, Lilla; Kerekes, Andrea; Bösze, Z.; Hiripi, Laszlo.

In: Journal of Biotechnology, Vol. 259, 10.10.2017, p. 86-90.

Research output: Article

Skoda, G, Hoffmann, OI, Gócza, E, Bodrogi, L, Kerekes, A, Bösze, Z & Hiripi, L 2017, 'Placenta-specific gene manipulation in rabbits', Journal of Biotechnology, vol. 259, pp. 86-90. https://doi.org/10.1016/j.jbiotec.2017.07.037
Skoda G, Hoffmann OI, Gócza E, Bodrogi L, Kerekes A, Bösze Z et al. Placenta-specific gene manipulation in rabbits. Journal of Biotechnology. 2017 okt. 10;259:86-90. https://doi.org/10.1016/j.jbiotec.2017.07.037
Skoda, Gabriella ; Hoffmann, Orsolya Ivett ; Gócza, Elen ; Bodrogi, Lilla ; Kerekes, Andrea ; Bösze, Z. ; Hiripi, Laszlo. / Placenta-specific gene manipulation in rabbits. In: Journal of Biotechnology. 2017 ; Vol. 259. pp. 86-90.
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