Pitfalls in hiv serology: Reagent-dependent changes in sensitivity and specificity of elisa kits

Eszter Ujhelyi, George Füst, Melinda Katonka, Maria Héjjas, Susan R. Hollán

Research output: Article

2 Citations (Scopus)

Abstract

Summary: Using confirmed positive and false-positive serum samples stored in deep frozen state we studied the reproducibility of the results obtained by different anti-HIV enzyme immunoassay (EIA) kits. Experiences obtained with 3 kits are presented. Two types of observations were made: (a) significant inter-lot, intra-lot and even inter-box sensitivity difference was found with some kits and (b) reactivity of the plates for true-positive and false-positive sera independently changed among different lots of the same kit: while reactivity for true positive sera was constant, a significant decrease or increase in reactivity for false-positive sera was found. These observations point to poor reproducibility of some commercial anti-HIV EIA kits that can cause serious difficulties in screening laboratories.

Original languageEnglish
Pages (from-to)208-211
Number of pages4
JournalJournal of Acquired Immune Deficiency Syndromes
Volume2
Issue number2
Publication statusPublished - ápr. 1989

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)

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