Summary: Using confirmed positive and false-positive serum samples stored in deep frozen state we studied the reproducibility of the results obtained by different anti-HIV enzyme immunoassay (EIA) kits. Experiences obtained with 3 kits are presented. Two types of observations were made: (a) significant inter-lot, intra-lot and even inter-box sensitivity difference was found with some kits and (b) reactivity of the plates for true-positive and false-positive sera independently changed among different lots of the same kit: while reactivity for true positive sera was constant, a significant decrease or increase in reactivity for false-positive sera was found. These observations point to poor reproducibility of some commercial anti-HIV EIA kits that can cause serious difficulties in screening laboratories.
|Number of pages||4|
|Journal||Journal of Acquired Immune Deficiency Syndromes|
|Publication status||Published - ápr. 1989|
ASJC Scopus subject areas
- Infectious Diseases
- Pharmacology (medical)