PIBF+ extracellular vesicles from mouse embryos affect IL-10 production by CD8+ cells

E. Pállinger, Zoltan Bognar, Agnes Bogdan, Timea Csabai, H. Ábrahám, J. Szekeres-Barthó

Research output: Article

8 Citations (Scopus)

Abstract

Earlier evidence suggests, that the embryo signals to the maternal immune system. Extracellular vesicles (EVs) are produced by all types of cells, and because they transport different kinds of molecules from one cell to the other, they can be considered as means of intercellular communication. The aim of this work was to test, whether the embryo is able to produce sufficient amounts of EVs to alter the function of peripheral lymphocytes. Embryo-derived EVs were identified by their Annexin V biding capacity, and sensitivity to Triton X dependent lysis, using flow cytometry. Transmission electron microscopy was used to detect EVs at the implantation site. Progesterone-induced blocking factor (PIBF) expression in embryo-derived EVs was demonstrated with immuno-electron microscopy. The % of IL-10 + murine lymphocytes was determined by flow cytometry. EVs were present in embryo culture media, but not in empty media. Mouse embryo-derived EVs adhere to the surface of both CD4+ and CD8+ murine peripheral T lymphocytes, partly, via phosphatidylserine binding. The number of IL-10+ murine peripheral CD8+ cells increases in the presence of embryo-derived EVS, and this effect is counteracted by pre-treatment of EVs with an anti-PIBF antibody, suggesting that the embryo communicates with the maternal immune system via EVs.

Original languageEnglish
Article number4662
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - dec. 1 2018

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Interleukin-10
Progesterone
Embryonic Structures
Immune System
Flow Cytometry
Mothers
Lymphocytes
blocking factor
Extracellular Vesicles
Blocking Antibodies
Immunoelectron Microscopy
Annexin A5
Phosphatidylserines
Transmission Electron Microscopy
Culture Media
T-Lymphocytes

ASJC Scopus subject areas

  • General

Cite this

PIBF+ extracellular vesicles from mouse embryos affect IL-10 production by CD8+ cells. / Pállinger, E.; Bognar, Zoltan; Bogdan, Agnes; Csabai, Timea; Ábrahám, H.; Szekeres-Barthó, J.

In: Scientific Reports, Vol. 8, No. 1, 4662, 01.12.2018.

Research output: Article

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