Phylogenetic analysis of metastatic progression in breast cancer using somatic mutations and copy number aberrations

David Brown, Dominiek Smeets, Borbála Székely, Denis Larsimont, A. Marcell Szász, Pierre Yves Adnet, Françoise Rothé, Ghizlane Rouas, Zsófia I. Nagy, Zsófia Faragó, Anna Mária Tökés, Magdolna Dank, Gyöngyvér Szentmártoni, Nóra Udvarhelyi, Gabriele Zoppoli, Lajos Pusztai, Martine Piccart, Janina Kulka, Diether Lambrechts, Christos SotiriouChristine Desmedt

Research output: Article

41 Citations (Scopus)

Abstract

Several studies using genome-wide molecular techniques have reported various degrees of genetic heterogeneity between primary tumours and their distant metastases. However, it has been difficult to discern patterns of dissemination owing to the limited number of patients and available metastases. Here, we use phylogenetic techniques on data generated using whole-exome sequencing and copy number profiling of primary and multiple-matched metastatic tumours from ten autopsied patients to infer the evolutionary history of breast cancer progression. We observed two modes of disease progression. In some patients, all distant metastases cluster on a branch separate from their primary lesion. Clonal frequency analyses of somatic mutations show that the metastases have a monoclonal origin and descend from a common 'metastatic precursor'. Alternatively, multiple metastatic lesions are seeded from different clones present within the primary tumour. We further show that a metastasis can be horizontally cross-seeded. These findings provide insights into breast cancer dissemination.

Original languageEnglish
Article number14944
JournalNature communications
Volume8
DOIs
Publication statusPublished - jan. 1 2017

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Brown, D., Smeets, D., Székely, B., Larsimont, D., Marcell Szász, A., Adnet, P. Y., Rothé, F., Rouas, G., Nagy, Z. I., Faragó, Z., Tökés, A. M., Dank, M., Szentmártoni, G., Udvarhelyi, N., Zoppoli, G., Pusztai, L., Piccart, M., Kulka, J., Lambrechts, D., ... Desmedt, C. (2017). Phylogenetic analysis of metastatic progression in breast cancer using somatic mutations and copy number aberrations. Nature communications, 8, [14944]. https://doi.org/10.1038/ncomms14944