Phosphorylation regulates the subcellular localization of Cucumber Mosaic Virus 2b protein

Katalin Nemes, Ákos Gellért, Asztéria Almási, Pál Vági, Réka Sáray, Katalin Kádár, K. Salánki

Research output: Article

6 Citations (Scopus)

Abstract

The 2b protein of Cucumber mosaic virus has a role in nearly all steps of the viral cycle including cell-to-cell movement, symptom induction and suppression of antiviral RNA silencing. Previous studies demonstrated the presence of 2b protein in the nucleus and in cytoplasm as well. Phosphorylation site of 2b protein is conserved in all CMV isolates, including proposed constitute motifs for casein kinase II and cyclin-dependent kinase 2. To discern the impact of 2b protein phosphorylation, we created eight different mutants to mimic the non-phosporylated (serine to alanine) as well as the phosphorylated state (serine to aspartic acid) of the protein. We compared these mutants to the wild-type (Rs-CMV) virus in terms of symptom induction, gene silencing suppressor activity as well as in cellular localization. Here, in this study we confirmed the phosphorylation of 2b protein in vivo, both in infected N. benthamiana and in infiltrated patches. Mutants containing aspartic acid in the phosphorylation site accumulated only in the cytoplasm indicating that phosphorylated 2b protein could not enter the nucleus. We identified a conserved dual phosphorylation switch in CMV 2b protein, which equilibrates the shuttling of the 2b protein between the nucleus and the cytoplasm, and regulates the suppressor activity of the 2b protein.

Original languageEnglish
Article number13444
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - dec. 1 2017

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Phosphorylation
Proteins
Cytoplasm
Aspartic Acid
Serine
Cyclin-Dependent Kinase 2
cucumber mosaic virus 2b protein
Casein Kinase II
Gene Silencing
RNA Interference
Alanine
Cell Movement
Antiviral Agents
Cell Cycle
Viruses

ASJC Scopus subject areas

  • General

Cite this

Phosphorylation regulates the subcellular localization of Cucumber Mosaic Virus 2b protein. / Nemes, Katalin; Gellért, Ákos; Almási, Asztéria; Vági, Pál; Sáray, Réka; Kádár, Katalin; Salánki, K.

In: Scientific Reports, Vol. 7, No. 1, 13444, 01.12.2017.

Research output: Article

Nemes, Katalin ; Gellért, Ákos ; Almási, Asztéria ; Vági, Pál ; Sáray, Réka ; Kádár, Katalin ; Salánki, K. / Phosphorylation regulates the subcellular localization of Cucumber Mosaic Virus 2b protein. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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