Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT

Emily Chen, Michael A. Kiebish, Justice McDaniel, Katarzyna Niedzwiecka, Roza Kucharczyk, Dora Ravasz, Fei Gao, Niven R. Narain, Rangaprasad Sarangarajan, Thomas N. Seyfried, V. Ádám-Vizi, C. Chinopoulos

Research output: Article

Abstract

Heterologous expression is a landmark technique for studying a protein itself or its effect on the expression host, in which membrane-embedded proteins are a common choice. Yet, the impact of inserting a foreign protein to the lipid environment of host membranes, has never been addressed. Here we demonstrated that heterologous expression of the Artemia franciscana adenine nucleotide translocase (ANT) in yeasts altered lipidomic composition of their inner mitochondrial membranes. Along with this, activities of complex II, IV and ATP synthase, all membrane-embedded components, were significantly decreased while their expression levels remained unaffected. Although the results represent an individual case of expressing a crustacean protein in yeast inner mitochondrial membranes, it cannot be excluded that host lipidome alterations is a more widespread epiphenomenon, potentially biasing heterologous expression experiments. Finally, our results raise the possibility that not only lipids modulate protein function, but also membrane-embedded proteins modulate lipid composition, thus revealing a reciprocal mode of regulation for these two biomolecular entities.

Original languageEnglish
Article number5915
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - dec. 1 2018

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ATP Translocases Mitochondrial ADP
Artemia
Membrane Proteins
Yeasts
Mitochondrial Membranes
Lipids
Arthropod Proteins
Dolphins
Proteins
Membranes
Adenosine Triphosphate

ASJC Scopus subject areas

  • General

Cite this

Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT. / Chen, Emily; Kiebish, Michael A.; McDaniel, Justice; Niedzwiecka, Katarzyna; Kucharczyk, Roza; Ravasz, Dora; Gao, Fei; Narain, Niven R.; Sarangarajan, Rangaprasad; Seyfried, Thomas N.; Ádám-Vizi, V.; Chinopoulos, C.

In: Scientific Reports, Vol. 8, No. 1, 5915, 01.12.2018.

Research output: Article

Chen, E, Kiebish, MA, McDaniel, J, Niedzwiecka, K, Kucharczyk, R, Ravasz, D, Gao, F, Narain, NR, Sarangarajan, R, Seyfried, TN, Ádám-Vizi, V & Chinopoulos, C 2018, 'Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT', Scientific Reports, vol. 8, no. 1, 5915. https://doi.org/10.1038/s41598-018-24305-2
Chen, Emily ; Kiebish, Michael A. ; McDaniel, Justice ; Niedzwiecka, Katarzyna ; Kucharczyk, Roza ; Ravasz, Dora ; Gao, Fei ; Narain, Niven R. ; Sarangarajan, Rangaprasad ; Seyfried, Thomas N. ; Ádám-Vizi, V. ; Chinopoulos, C. / Perturbation of the yeast mitochondrial lipidome and associated membrane proteins following heterologous expression of Artemia-ANT. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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