Pepper 9- and 13-lipoxygenase genes are differentially activated by two tobamoviruses and by hormone treatments

Csilla Juhász, István Tóbiás, A. Ádám, G. Kátay, G. Gullner

Research output: Article

5 Citations (Scopus)

Abstract

Two novel pepper 13-lipoxygenase (LOX) genes were cloned and their expressions were compared with those of three 9- LOX genes in pepper leaves inoculated with two different tobamoviruses. Obuda pepper virus (ObPV) inoculation led to a massive induction of pathogenesis-related genes and to the development of hypersensitive reaction (incompatible interaction), while Pepper mild mottle virus (PMMoV) inoculation resulted in a compatible interaction. Both virus infections markedly activated the expression of the two novel 13-LOXs. The magnitudes of induction of 13- LOXs did not differ substantially between the ObPV- and PMMoV-inoculated leaves. The induction of three 9-LOXs was markedly more robust and rapid in ObPV-inoculated leaves than in PMMoV-inoculated ones. LOXs were very differentially activated in pepper leaves treated with defense hormones. A large number of hormone-related cis-regulatory elements were identified in the promoter regions of LOXs. ObPV inoculation resulted also in the substantial up-regulation of an omega-6-fatty acid desaturase gene. Our results suggest that 9-LOX-dependent pathways are more probably involved in the suppression of virus replication than 13-LOX-dependent plant responses.

Original languageEnglish
Article number1132
Pages (from-to)59-69
Number of pages11
JournalPhysiological and Molecular Plant Pathology
Volume92
DOIs
Publication statusPublished - okt. 1 2015

Fingerprint

Obuda pepper virus
Tobamovirus
Pepper mild mottle virus
lipoxygenase
pepper
hormones
Hormones
Viruses
Genes
leaves
genes
Lipoxygenase
stearoyl-CoA desaturase
regulatory sequences
omega-6 fatty acids
hypersensitive response
virus replication
plant response
Omega-6 Fatty Acids
Fatty Acid Desaturases

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

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title = "Pepper 9- and 13-lipoxygenase genes are differentially activated by two tobamoviruses and by hormone treatments",
abstract = "Two novel pepper 13-lipoxygenase (LOX) genes were cloned and their expressions were compared with those of three 9- LOX genes in pepper leaves inoculated with two different tobamoviruses. Obuda pepper virus (ObPV) inoculation led to a massive induction of pathogenesis-related genes and to the development of hypersensitive reaction (incompatible interaction), while Pepper mild mottle virus (PMMoV) inoculation resulted in a compatible interaction. Both virus infections markedly activated the expression of the two novel 13-LOXs. The magnitudes of induction of 13- LOXs did not differ substantially between the ObPV- and PMMoV-inoculated leaves. The induction of three 9-LOXs was markedly more robust and rapid in ObPV-inoculated leaves than in PMMoV-inoculated ones. LOXs were very differentially activated in pepper leaves treated with defense hormones. A large number of hormone-related cis-regulatory elements were identified in the promoter regions of LOXs. ObPV inoculation resulted also in the substantial up-regulation of an omega-6-fatty acid desaturase gene. Our results suggest that 9-LOX-dependent pathways are more probably involved in the suppression of virus replication than 13-LOX-dependent plant responses.",
keywords = "Defense hormone, Desaturase, Lipoxygenase, Pepper, Tobamovirus",
author = "Csilla Juh{\'a}sz and Istv{\'a}n T{\'o}bi{\'a}s and A. {\'A}d{\'a}m and G. K{\'a}tay and G. Gullner",
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T1 - Pepper 9- and 13-lipoxygenase genes are differentially activated by two tobamoviruses and by hormone treatments

AU - Juhász, Csilla

AU - Tóbiás, István

AU - Ádám, A.

AU - Kátay, G.

AU - Gullner, G.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Two novel pepper 13-lipoxygenase (LOX) genes were cloned and their expressions were compared with those of three 9- LOX genes in pepper leaves inoculated with two different tobamoviruses. Obuda pepper virus (ObPV) inoculation led to a massive induction of pathogenesis-related genes and to the development of hypersensitive reaction (incompatible interaction), while Pepper mild mottle virus (PMMoV) inoculation resulted in a compatible interaction. Both virus infections markedly activated the expression of the two novel 13-LOXs. The magnitudes of induction of 13- LOXs did not differ substantially between the ObPV- and PMMoV-inoculated leaves. The induction of three 9-LOXs was markedly more robust and rapid in ObPV-inoculated leaves than in PMMoV-inoculated ones. LOXs were very differentially activated in pepper leaves treated with defense hormones. A large number of hormone-related cis-regulatory elements were identified in the promoter regions of LOXs. ObPV inoculation resulted also in the substantial up-regulation of an omega-6-fatty acid desaturase gene. Our results suggest that 9-LOX-dependent pathways are more probably involved in the suppression of virus replication than 13-LOX-dependent plant responses.

AB - Two novel pepper 13-lipoxygenase (LOX) genes were cloned and their expressions were compared with those of three 9- LOX genes in pepper leaves inoculated with two different tobamoviruses. Obuda pepper virus (ObPV) inoculation led to a massive induction of pathogenesis-related genes and to the development of hypersensitive reaction (incompatible interaction), while Pepper mild mottle virus (PMMoV) inoculation resulted in a compatible interaction. Both virus infections markedly activated the expression of the two novel 13-LOXs. The magnitudes of induction of 13- LOXs did not differ substantially between the ObPV- and PMMoV-inoculated leaves. The induction of three 9-LOXs was markedly more robust and rapid in ObPV-inoculated leaves than in PMMoV-inoculated ones. LOXs were very differentially activated in pepper leaves treated with defense hormones. A large number of hormone-related cis-regulatory elements were identified in the promoter regions of LOXs. ObPV inoculation resulted also in the substantial up-regulation of an omega-6-fatty acid desaturase gene. Our results suggest that 9-LOX-dependent pathways are more probably involved in the suppression of virus replication than 13-LOX-dependent plant responses.

KW - Defense hormone

KW - Desaturase

KW - Lipoxygenase

KW - Pepper

KW - Tobamovirus

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