PEGylation of surfacted magnetite core-shell nanoparticles for biomedical application

E. Illés, M. Szekeres, Edina Kupcsik, Ildiko´ Y. To´th, Katalin Farkas, Ange´la Jedlovszky-Hajdu´, E. Tombácz

Research output: Article

37 Citations (Scopus)

Abstract

The surface of oleate double layer coated (surfacted) magnetite nanoparticles (OA@MNPs) was coated by PEG (poly(ethylene glycol) of Mw=1000, 4000 or 20,000Da, respectively, to get core-shell structured nanomagnets. The oleate bilayers were prepared in two different ways; (i) oleic acid was added directly into the magnetite co-precipitation mixture containing the MNPs to obtain OA@s-MNP samples - s-MNP standing for "as-synthesized MNP" and (ii) sodium oleate (oleate anion, OA) was added to the purified MNPs to obtain OA@p-MNP samples - p-MNP standing for "purified MNP". The effect of the surfactant addition method on the pH- and ionic strength-dependent stability (dynamic laser light scattering and laser-Doppler electrophoresis experiments), the biomedical applicability (MRI measurements) and the biocompatibility (blood sedimentation and blood smear tests) of the core-shell MNPs was studied. Different mechanisms of oleate adsorption were found in ATR FT-IR experiments (inner sphere surface complexation via ligand exchange for the s-MNPs and additional H-bonding for the p-MNPs), suggesting different behaviour. The colloidal stability and salt tolerance of the two kinds of OA@MNPs were similar, but the hydrodynamic diameter of the OA@s-MNP was considerably larger than that of OA@p-MNP. In accordance with this, the r2 relaxation was also higher for the s-MNP samples (~400 and ~200mM-1s-1, respectively). The physico-chemical tests indicate that the OA-coated MNPs form clusters and the degree of clustering of OA@s-MNPs is significantly greater than that of OA@p-MPNs. PEGylation does not appear to affect colloidal stability and salt tolerance meaningfully. The adsorption of PEG was proved experimentally. We have found that the PEG top layer decreases the electrostatic contribution, nevertheless increases the steric contribution of the original electrosteric stabilization caused by the OA double layer. However, an increase in the molecular weight above 1000Da and the amount of added PEG above 5mmol/g gradually reduces the salt tolerance of the samples. The results indicate strong potential for biomedical application and biocompatibility of the PEGylated MNPs.

Original languageEnglish
Pages (from-to)429-440
Number of pages12
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume460
DOIs
Publication statusPublished - jan. 26 2014

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Ferrosoferric Oxide
Magnetite
Oleic Acid
magnetite
Negative ions
Nanoparticles
anions
Anions
nanoparticles
Polyethylene glycols
biocompatibility
Salts
salts
Biocompatibility
blood
Blood
chemical tests
Magnetite nanoparticles
Adsorption
dynamic stability

ASJC Scopus subject areas

  • Colloid and Surface Chemistry

Cite this

PEGylation of surfacted magnetite core-shell nanoparticles for biomedical application. / Illés, E.; Szekeres, M.; Kupcsik, Edina; To´th, Ildiko´ Y.; Farkas, Katalin; Jedlovszky-Hajdu´, Ange´la; Tombácz, E.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 460, 26.01.2014, p. 429-440.

Research output: Article

Illés, E. ; Szekeres, M. ; Kupcsik, Edina ; To´th, Ildiko´ Y. ; Farkas, Katalin ; Jedlovszky-Hajdu´, Ange´la ; Tombácz, E. / PEGylation of surfacted magnetite core-shell nanoparticles for biomedical application. In: Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2014 ; Vol. 460. pp. 429-440.
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AU - Szekeres, M.

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AU - Farkas, Katalin

AU - Jedlovszky-Hajdu´, Ange´la

AU - Tombácz, E.

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N2 - The surface of oleate double layer coated (surfacted) magnetite nanoparticles (OA@MNPs) was coated by PEG (poly(ethylene glycol) of Mw=1000, 4000 or 20,000Da, respectively, to get core-shell structured nanomagnets. The oleate bilayers were prepared in two different ways; (i) oleic acid was added directly into the magnetite co-precipitation mixture containing the MNPs to obtain OA@s-MNP samples - s-MNP standing for "as-synthesized MNP" and (ii) sodium oleate (oleate anion, OA) was added to the purified MNPs to obtain OA@p-MNP samples - p-MNP standing for "purified MNP". The effect of the surfactant addition method on the pH- and ionic strength-dependent stability (dynamic laser light scattering and laser-Doppler electrophoresis experiments), the biomedical applicability (MRI measurements) and the biocompatibility (blood sedimentation and blood smear tests) of the core-shell MNPs was studied. Different mechanisms of oleate adsorption were found in ATR FT-IR experiments (inner sphere surface complexation via ligand exchange for the s-MNPs and additional H-bonding for the p-MNPs), suggesting different behaviour. The colloidal stability and salt tolerance of the two kinds of OA@MNPs were similar, but the hydrodynamic diameter of the OA@s-MNP was considerably larger than that of OA@p-MNP. In accordance with this, the r2 relaxation was also higher for the s-MNP samples (~400 and ~200mM-1s-1, respectively). The physico-chemical tests indicate that the OA-coated MNPs form clusters and the degree of clustering of OA@s-MNPs is significantly greater than that of OA@p-MPNs. PEGylation does not appear to affect colloidal stability and salt tolerance meaningfully. The adsorption of PEG was proved experimentally. We have found that the PEG top layer decreases the electrostatic contribution, nevertheless increases the steric contribution of the original electrosteric stabilization caused by the OA double layer. However, an increase in the molecular weight above 1000Da and the amount of added PEG above 5mmol/g gradually reduces the salt tolerance of the samples. The results indicate strong potential for biomedical application and biocompatibility of the PEGylated MNPs.

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