PEGylation of superparamagnetic iron oxide nanoparticles with self-organizing polyacrylate-PEG brushes for contrast enhancement in MRI diagnosis

E. Illés, M. Szekeres, Ildikó Y. Tóth, Katalin Farkas, I. Földesi, Ákos Szabó, B. Iván, E. Tombácz

Research output: Article

7 Citations (Scopus)

Abstract

For biomedical applications, superparamagnetic nanoparticles (MNPs) have to be coated with a stealth layer that provides colloidal stability in biological media, long enough persistence and circulation times for reaching the expected medical aims, and anchor sites for further attachment of bioactive agents. One of such stealth molecules designed and synthesized by us, poly(polyethylene glycol methacrylate-co-acrylic acid) referred to as P(PEGMA-AA), was demonstrated to make MNPs reasonably resistant to cell internalization, and be an excellent candidate for magnetic hyperthermia treatments in addition to possessing the necessary colloidal stability under physiological conditions (Illés et al. J. Magn. Magn. Mater. 2018, 451, 710–720). In the present work, we elaborated on the molecular background of the formation of the P(PEGMA-AA)-coated MNPs, and of their remarkable colloidal stability and salt tolerance by using potentiometric acid–base titration, adsorption isotherm determination, infrared spectroscopy (FT-IR ATR), dynamic light scattering, and electrokinetic potential determination methods. The P(PEGMA-AA)@MNPs have excellent blood compatibility as demonstrated in blood sedimentation, smears, and white blood cell viability experiments. In addition, blood serum proteins formed a protein corona, protecting the particles against aggregation (found in dynamic light scattering and electrokinetic potential measurements). Our novel particles also proved to be promising candidates for MRI diagnosis, exhibiting one of the highest values of r2 relaxivity (451 mM−1 s−1) found in literature.

Original languageEnglish
Article number776
JournalNanomaterials
Volume8
Issue number10
DOIs
Publication statusPublished - okt. 1 2018

Fingerprint

Brushes
Polyacrylates
Iron oxides
Magnetic resonance imaging
Polyethylene glycols
Blood
Nanoparticles
Acrylics
Dynamic light scattering
Acids
Proteins
Anchors
Titration
Adsorption isotherms
Sedimentation
Blood Proteins
Infrared spectroscopy
Agglomeration
Salts
Cells

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

Cite this

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title = "PEGylation of superparamagnetic iron oxide nanoparticles with self-organizing polyacrylate-PEG brushes for contrast enhancement in MRI diagnosis",
abstract = "For biomedical applications, superparamagnetic nanoparticles (MNPs) have to be coated with a stealth layer that provides colloidal stability in biological media, long enough persistence and circulation times for reaching the expected medical aims, and anchor sites for further attachment of bioactive agents. One of such stealth molecules designed and synthesized by us, poly(polyethylene glycol methacrylate-co-acrylic acid) referred to as P(PEGMA-AA), was demonstrated to make MNPs reasonably resistant to cell internalization, and be an excellent candidate for magnetic hyperthermia treatments in addition to possessing the necessary colloidal stability under physiological conditions (Ill{\'e}s et al. J. Magn. Magn. Mater. 2018, 451, 710–720). In the present work, we elaborated on the molecular background of the formation of the P(PEGMA-AA)-coated MNPs, and of their remarkable colloidal stability and salt tolerance by using potentiometric acid–base titration, adsorption isotherm determination, infrared spectroscopy (FT-IR ATR), dynamic light scattering, and electrokinetic potential determination methods. The P(PEGMA-AA)@MNPs have excellent blood compatibility as demonstrated in blood sedimentation, smears, and white blood cell viability experiments. In addition, blood serum proteins formed a protein corona, protecting the particles against aggregation (found in dynamic light scattering and electrokinetic potential measurements). Our novel particles also proved to be promising candidates for MRI diagnosis, exhibiting one of the highest values of r2 relaxivity (451 mM−1 s−1) found in literature.",
keywords = "Blood compatibility, Colloidal stability, Core, MRI contrast agents, PEG coating, Shell nanoparticles, Superparamagnetic nanoparticles",
author = "E. Ill{\'e}s and M. Szekeres and T{\'o}th, {Ildik{\'o} Y.} and Katalin Farkas and I. F{\"o}ldesi and {\'A}kos Szab{\'o} and B. Iv{\'a}n and E. Tomb{\'a}cz",
year = "2018",
month = "10",
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language = "English",
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T1 - PEGylation of superparamagnetic iron oxide nanoparticles with self-organizing polyacrylate-PEG brushes for contrast enhancement in MRI diagnosis

AU - Illés, E.

AU - Szekeres, M.

AU - Tóth, Ildikó Y.

AU - Farkas, Katalin

AU - Földesi, I.

AU - Szabó, Ákos

AU - Iván, B.

AU - Tombácz, E.

PY - 2018/10/1

Y1 - 2018/10/1

N2 - For biomedical applications, superparamagnetic nanoparticles (MNPs) have to be coated with a stealth layer that provides colloidal stability in biological media, long enough persistence and circulation times for reaching the expected medical aims, and anchor sites for further attachment of bioactive agents. One of such stealth molecules designed and synthesized by us, poly(polyethylene glycol methacrylate-co-acrylic acid) referred to as P(PEGMA-AA), was demonstrated to make MNPs reasonably resistant to cell internalization, and be an excellent candidate for magnetic hyperthermia treatments in addition to possessing the necessary colloidal stability under physiological conditions (Illés et al. J. Magn. Magn. Mater. 2018, 451, 710–720). In the present work, we elaborated on the molecular background of the formation of the P(PEGMA-AA)-coated MNPs, and of their remarkable colloidal stability and salt tolerance by using potentiometric acid–base titration, adsorption isotherm determination, infrared spectroscopy (FT-IR ATR), dynamic light scattering, and electrokinetic potential determination methods. The P(PEGMA-AA)@MNPs have excellent blood compatibility as demonstrated in blood sedimentation, smears, and white blood cell viability experiments. In addition, blood serum proteins formed a protein corona, protecting the particles against aggregation (found in dynamic light scattering and electrokinetic potential measurements). Our novel particles also proved to be promising candidates for MRI diagnosis, exhibiting one of the highest values of r2 relaxivity (451 mM−1 s−1) found in literature.

AB - For biomedical applications, superparamagnetic nanoparticles (MNPs) have to be coated with a stealth layer that provides colloidal stability in biological media, long enough persistence and circulation times for reaching the expected medical aims, and anchor sites for further attachment of bioactive agents. One of such stealth molecules designed and synthesized by us, poly(polyethylene glycol methacrylate-co-acrylic acid) referred to as P(PEGMA-AA), was demonstrated to make MNPs reasonably resistant to cell internalization, and be an excellent candidate for magnetic hyperthermia treatments in addition to possessing the necessary colloidal stability under physiological conditions (Illés et al. J. Magn. Magn. Mater. 2018, 451, 710–720). In the present work, we elaborated on the molecular background of the formation of the P(PEGMA-AA)-coated MNPs, and of their remarkable colloidal stability and salt tolerance by using potentiometric acid–base titration, adsorption isotherm determination, infrared spectroscopy (FT-IR ATR), dynamic light scattering, and electrokinetic potential determination methods. The P(PEGMA-AA)@MNPs have excellent blood compatibility as demonstrated in blood sedimentation, smears, and white blood cell viability experiments. In addition, blood serum proteins formed a protein corona, protecting the particles against aggregation (found in dynamic light scattering and electrokinetic potential measurements). Our novel particles also proved to be promising candidates for MRI diagnosis, exhibiting one of the highest values of r2 relaxivity (451 mM−1 s−1) found in literature.

KW - Blood compatibility

KW - Colloidal stability

KW - Core

KW - MRI contrast agents

KW - PEG coating

KW - Shell nanoparticles

KW - Superparamagnetic nanoparticles

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DO - 10.3390/nano8100776

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