Partial protection by poly(ADP-ribose) polymerase inhibitors from nitroxyl-induced cytotoxicity in thymocytes

P. Bai, Edina Bakondi, Éva Szabó, P. Gergely, Csaba Szabó, L. Virag

Research output: Article

40 Citations (Scopus)

Abstract

Nitroxyl (NO-/HNO), has been proposed to be one of the NO-derived cytotoxic species. Although the biological effect of nitroxyl is largely unknown, it has been reported to cause DNA breakage and cytotoxicity. We have therefore investigated whether NO-/HNO-induced DNA single-strand breakage activates the nuclear nick sensor enzyme poly(ADP-ribose) polymerase (PARP) and whether PARP activation affects the mode of NO-/HNO- induced cell death. NO-/HNO generated from Angeli's salt (AS, sodium trioxodinitrate) (0-300 μM) induced DNA single-strand breakage, PARP activation, and a concentration-dependent cytotoxicity in murine thymocytes. AS-induced cell death was also accompanied by decreased mitochondrial membrane potential and increased secondary superoxide production. The cytotoxicity of AS, as measured by propidium iodide uptake, was abolished by electron acceptors potassium ferricyanide, TEMPOL, the intracellular calcium chelator BAPTA-AM, and by PARP inhibitors 3-aminobenzamide (3-AB) and PJ-34. The cytoprotective effect of 3-AB was paralleled by increased output of AS-induced apoptotic parameters such as phosphatidylserine exposure, caspase activation, and DNA fragmentation. No significant increase in tyrosine nitration could be observed in AS-treated thymocytes as opposed to peroxynitrite-treated cells, indicating that tyrosine nitration is not likely to contribute to NO-/HNO-induced cytotoxicity. Our results demonstrate that NO-/HNO-induced PARP activation shifts the default apoptotic cell death toward necrosis in thymocytes. However, as total PARP inhibition resulted only in 30% cytoprotection, PARP-independent mechanisms dominate NO-/HNO-induced cytotoxicity in thymocytes.

Original languageEnglish
Pages (from-to)1616-1623
Number of pages8
JournalFree Radical Biology and Medicine
Volume31
Issue number12
DOIs
Publication statusPublished - dec. 15 2001

Fingerprint

Poly(ADP-ribose) Polymerases
Thymocytes
Cytotoxicity
Cell death
Chemical activation
Nitration
Cell Death
DNA
Tyrosine
Enzyme sensors
Peroxynitrous Acid
Cytoprotection
Propidium
Mitochondrial Membrane Potential
Phosphatidylserines
DNA Fragmentation
Caspases
Superoxides
nitroxyl
Poly(ADP-ribose) Polymerase Inhibitors

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Partial protection by poly(ADP-ribose) polymerase inhibitors from nitroxyl-induced cytotoxicity in thymocytes. / Bai, P.; Bakondi, Edina; Szabó, Éva; Gergely, P.; Szabó, Csaba; Virag, L.

In: Free Radical Biology and Medicine, Vol. 31, No. 12, 15.12.2001, p. 1616-1623.

Research output: Article

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AU - Bai, P.

AU - Bakondi, Edina

AU - Szabó, Éva

AU - Gergely, P.

AU - Szabó, Csaba

AU - Virag, L.

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AB - Nitroxyl (NO-/HNO), has been proposed to be one of the NO•-derived cytotoxic species. Although the biological effect of nitroxyl is largely unknown, it has been reported to cause DNA breakage and cytotoxicity. We have therefore investigated whether NO-/HNO-induced DNA single-strand breakage activates the nuclear nick sensor enzyme poly(ADP-ribose) polymerase (PARP) and whether PARP activation affects the mode of NO-/HNO- induced cell death. NO-/HNO generated from Angeli's salt (AS, sodium trioxodinitrate) (0-300 μM) induced DNA single-strand breakage, PARP activation, and a concentration-dependent cytotoxicity in murine thymocytes. AS-induced cell death was also accompanied by decreased mitochondrial membrane potential and increased secondary superoxide production. The cytotoxicity of AS, as measured by propidium iodide uptake, was abolished by electron acceptors potassium ferricyanide, TEMPOL, the intracellular calcium chelator BAPTA-AM, and by PARP inhibitors 3-aminobenzamide (3-AB) and PJ-34. The cytoprotective effect of 3-AB was paralleled by increased output of AS-induced apoptotic parameters such as phosphatidylserine exposure, caspase activation, and DNA fragmentation. No significant increase in tyrosine nitration could be observed in AS-treated thymocytes as opposed to peroxynitrite-treated cells, indicating that tyrosine nitration is not likely to contribute to NO-/HNO-induced cytotoxicity. Our results demonstrate that NO-/HNO-induced PARP activation shifts the default apoptotic cell death toward necrosis in thymocytes. However, as total PARP inhibition resulted only in 30% cytoprotection, PARP-independent mechanisms dominate NO-/HNO-induced cytotoxicity in thymocytes.

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KW - Free radicals

KW - Necrosis

KW - Nitric oxide

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