PACAP is transiently expressed in anterior pituitary gland of rats: In situ hybridization and cell immunoblot assay studies

Andrea Heinzlmann, Eszter Kirilly, Kinga Meltzer, Eniko Szabó, Akemichi Baba, Hitoshi Hashimoto, K. Köves

Research output: Article

12 Citations (Scopus)

Abstract

In this work the expression of PACAP (pituitary adenylate cyclase activating polypeptide) in rat anterior pituitary was demonstrated for the first time using in situ hybridization. The number of cells showing PACAP signal in intact male rats was negligible similarly to that of diestrous rats. In proestrous rats sacrificed at 10 h there was a moderate increase in the expression and after a decrease at 16 h and 18 h, there was a transient peak at 20 h and then the number of labeled cells was declined again (22 h). In the cell immunoblot assay study it was observed that the number of PACAP blot forming (PACAP releasing) cells in an anterior pituitary cell culture changed according to a similar pattern as the number of PACAP expressing cells. The number of blots was also the highest when the animals were sacrificed in the evening of proestrus at 20 h. The results obtained by in situ hybridization and cell immunoblot assay well correlate with each other. The above-mentioned results support our hypothesis that the enhanced expression and secretion of PACAP in the pituitary gland may be involved in ceasing the LH surge.

Original languageEnglish
Pages (from-to)571-577
Number of pages7
JournalPeptides
Volume29
Issue number4
DOIs
Publication statusPublished - ápr. 2008

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Pituitary Adenylate Cyclase-Activating Polypeptide
Anterior Pituitary Gland
In Situ Hybridization
Rats
Assays
Cell Count
Proestrus
Pituitary Gland
Cell culture
Animals
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

PACAP is transiently expressed in anterior pituitary gland of rats : In situ hybridization and cell immunoblot assay studies. / Heinzlmann, Andrea; Kirilly, Eszter; Meltzer, Kinga; Szabó, Eniko; Baba, Akemichi; Hashimoto, Hitoshi; Köves, K.

In: Peptides, Vol. 29, No. 4, 04.2008, p. 571-577.

Research output: Article

Heinzlmann, Andrea ; Kirilly, Eszter ; Meltzer, Kinga ; Szabó, Eniko ; Baba, Akemichi ; Hashimoto, Hitoshi ; Köves, K. / PACAP is transiently expressed in anterior pituitary gland of rats : In situ hybridization and cell immunoblot assay studies. In: Peptides. 2008 ; Vol. 29, No. 4. pp. 571-577.
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AU - Kirilly, Eszter

AU - Meltzer, Kinga

AU - Szabó, Eniko

AU - Baba, Akemichi

AU - Hashimoto, Hitoshi

AU - Köves, K.

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KW - Ovarian cyclicity

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