Orientation of the genetic and physical map of Rhizobium meliloti temperate phage 16-3

László Dorgai, F. Olasz, Mária Berényi, Géza Dallmann, Anikó Páy, L. Orosz

Research output: Article

17 Citations (Scopus)

Abstract

The attachment site, the C cistron of Rhizobium meliloti temperate phage 16-3, and the insertion of the host cys46+ gene in the phage genome were localized on the HindIII and EcoRJ restriction endonuclease maps, as well as mapped genetically. The strategy employed included restriction analysis and Southern in situ hybridization of plasmid pGY1, which carries the bacterial chromosome region containing the integration site of 16-3, plasmid pGY2, which carries the 16-3 prophage, deletion and inversion mutants, and the cys46+ transducing 16-3 particles. The colinear array of genetic and physical data was possible. The possibility of isolation of a replacement phage vector for Rhizobium is discussed.

Original languageEnglish
Pages (from-to)321-325
Number of pages5
JournalMGG Molecular & General Genetics
Volume182
Issue number2
DOIs
Publication statusPublished - júl. 1981

Fingerprint

Sinorhizobium meliloti
Bacteriophages
Plasmids
Bacterial Chromosomes
Prophages
Rhizobium
DNA Restriction Enzymes
Genes
In Situ Hybridization
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Orientation of the genetic and physical map of Rhizobium meliloti temperate phage 16-3. / Dorgai, László; Olasz, F.; Berényi, Mária; Dallmann, Géza; Páy, Anikó; Orosz, L.

In: MGG Molecular & General Genetics, Vol. 182, No. 2, 07.1981, p. 321-325.

Research output: Article

Dorgai, László ; Olasz, F. ; Berényi, Mária ; Dallmann, Géza ; Páy, Anikó ; Orosz, L. / Orientation of the genetic and physical map of Rhizobium meliloti temperate phage 16-3. In: MGG Molecular & General Genetics. 1981 ; Vol. 182, No. 2. pp. 321-325.
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