Optical waveguide lightmode spectroscopy technique-based immunosensor development for aflatoxin B1 determination in spice paprika samples

Krisztina Majer-Baranyi, Zsolt Zalán, Mária Mörtl, Judit Juracsek, István Szendro, A. Székács, N. Adányi

Research output: Article

13 Citations (Scopus)

Abstract

Optical waveguide lightmode spectroscopy (OWLS) technique has been applied to label-free detection of aflatoxin B1 in a competitive immunoassay format, with the aim to compare the analytical goodness of the developed OWLS immunosenor with HPLC and enzyme-linked immunosorbent assay (ELISA) methods for the detection of aflatoxin in spice paprika matrix. We have also assessed applicability of the QuEChERS method prior to ELISA measurements, and the results were compared to those obtained by traditional solvent extraction followed by immunoaffinity clean-up. The AFB1 content of sixty commercial spice paprika samples from different countries were measured with the developed and optimized OWLS immunosensor. Comparing the results from the indirect immunosensor to that obtained by HPLC or ELISA provided excellent correlation (with regression coefficients above 0.94) indicating that the competitive OWLS immunosensor has a potential for quick determination of aflatoxin B1 in paprika samples.

Original languageEnglish
Pages (from-to)972-977
Number of pages6
JournalFood Chemistry
Volume211
DOIs
Publication statusPublished - nov. 15 2016

Fingerprint

Immunosensors
paprika
Capsicum
Spices
Aflatoxin B1
aflatoxin B1
Optical waveguides
spices
Immunosorbents
spectroscopy
Spectrum Analysis
Spectroscopy
Assays
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Enzymes
High Pressure Liquid Chromatography
sampling
Aflatoxins
Solvent extraction

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Optical waveguide lightmode spectroscopy technique-based immunosensor development for aflatoxin B1 determination in spice paprika samples. / Majer-Baranyi, Krisztina; Zalán, Zsolt; Mörtl, Mária; Juracsek, Judit; Szendro, István; Székács, A.; Adányi, N.

In: Food Chemistry, Vol. 211, 15.11.2016, p. 972-977.

Research output: Article

Majer-Baranyi, Krisztina ; Zalán, Zsolt ; Mörtl, Mária ; Juracsek, Judit ; Szendro, István ; Székács, A. ; Adányi, N. / Optical waveguide lightmode spectroscopy technique-based immunosensor development for aflatoxin B1 determination in spice paprika samples. In: Food Chemistry. 2016 ; Vol. 211. pp. 972-977.
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AU - Juracsek, Judit

AU - Szendro, István

AU - Székács, A.

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