Optical waveguide light-mode spectroscopy immunosensors for environmental monitoring

A. Székács, N. Adányi, Inna Székács, Krisztina Majer-Baranyi, István Szendro

Research output: Article

32 Citations (Scopus)

Abstract

Coupling the high specificity of the immunoanalytical reaction with the high sensitivity of optical waveguide light-mode spectroscopy (OWLS) detection gives the possibility to develop immunosensors with in most cases a definitely lower detection limit than traditionally used immunoassays. Measurements were performed on the sensitized surface of optical waveguide grating coupler sensors (2400 lines/mm grating). The OWLS technique is based on the precise measurement of the resonance angle of a polarized laser light (632.8 nm), diffracted by a grating and incoupled into a thin waveguide. The effective refractive index, determined from the resonance incoupling angle detected at high accuracy, allows determination of layer thickness and coverage (or mass) of the adsorbed or bound material with ultrahigh sensitivity. OWLS immunosensors were developed as label-free immunosensors with an amino group modified SiO2-TiO 2 sensor surface on which the immunoreactants could be anchored. One of the components of the antibody-antigen complex was chemically bound on the sensor surface, allowing noncompetitive or competitive detection of the analytes. To illustrate that the resulting immunosensors are suitable for the determination of small and large molecular weight analytes, OWLS sensor formats were applied for quantitative detection of a herbicide active ingredient trifluralin, a Fusarium mycotoxin zearalenone, and an egg yolk protein of key importance in endocrine regulation, vitellogenin.

Original languageEnglish
JournalApplied Optics
Volume48
Issue number4
DOIs
Publication statusPublished - febr. 1 2009

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Immunosensors
environmental monitoring
Optical waveguides
optical waveguides
Spectroscopy
Monitoring
spectroscopy
sensors
Sensors
gratings
herbicides
immunoassay
Herbicides
eggs
antigens
Antigens
antibodies
Antibodies
ingredients
couplers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Optical waveguide light-mode spectroscopy immunosensors for environmental monitoring. / Székács, A.; Adányi, N.; Székács, Inna; Majer-Baranyi, Krisztina; Szendro, István.

In: Applied Optics, Vol. 48, No. 4, 01.02.2009.

Research output: Article

Székács, A. ; Adányi, N. ; Székács, Inna ; Majer-Baranyi, Krisztina ; Szendro, István. / Optical waveguide light-mode spectroscopy immunosensors for environmental monitoring. In: Applied Optics. 2009 ; Vol. 48, No. 4.
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