Introduction The main goal of cryopreservation of cells, gametes, or embryos is to preserve the specimen in a state of suspended animation, with the hope that it can be reanimated after a certain period of time to continue its normal development. Cryopreservation procedures are designed to minimize damages caused by formation and growth of ice crystals ; however, the challenge of a successful cryopreservation is to be able to cool and recover cells from the ultra-low temperatures at which no changes in metabolism and structures are stable over time. Cryopreservation occurs when cryoprotectants or antifreeze solutions are added to the solution surrounding the cell, and then cooled at a certain rate. The biophysical changes caused by the transition of water to ice during cooling are the main cause of damage rather than the low temperature per se. As ice crystals grow, there is a significant osmotic stress, this “freeze-dehydration” was one of the first harmful consequences identified in cell cryobiology that causes several hazardous events including changes in ultrastructure of cell membranes, loss or fusion of membranes, and organelle disruption. In order to avoid cryo-damages, the cooling and warming protocol must be tailored according to the cell characteristics. Therefore, the compositions and modifications of cryomedia are very important to the success of cryopreservation.
|Title of host publication||Culture Media, Solutions, and Systems in Human ART|
|Publisher||Cambridge University Press|
|Number of pages||11|
|Publication status||Published - jan. 1 2014|
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